A portable microfluidic platform for rapid determination of microbial load and somatic cell count in milk
Makaleler > Tam Makale · 2019
Yazarlar: Gamze Duven, Barbaros Cetin, Huseyin Kurtuldu, Gülten GÜNDÜZ, Şebnem TAVMAN, Duygu KIŞLA
Biomedical Microdevices, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2019, cilt 21, ss 49-49
DOI: 10.1007/s10544-019-0407-8
Özet
Microfluidics systems that have been emerged in the last 20years and used for processing the fluid in a microchannel structure at microliter levels are alternative to the conventional methods. The objective of the study is to develop a microfluidic platform for determination of the microbial load and the number of somatic cells in milk. For this purpose, a polydimethylsiloxane (PDMS) chip with a channel size of 300mx60m was produced. Cells/bacteria labeled with fluorescent stain in milk were counted with the proposed microfluidic platform and the results were compared with the reference cell concentration/the bacterial counts by conventional method. It was found that our platform could count somatic and bacterial cells with an accuracy above 80% in 20min run for each analysis. The portable overall platform has an overall dimension of 25x25x25 cm and weighs approximately 9kg.