Biological activities of Turkish Alkanna species
Makaleler > Tam Makale · 2009
Yazarlar: C Sevimli-Gür, E Bedir, S Bıçkıcı, S Nalbantoğlu, SG Şenol, Tamer KARAYILDIRIM, Özgen ALANKUŞ, İ Deliloğlu-Gürhan, Kemal Sami KORKMAZ
Planta Medica, Uluslararası, Wos, 2009, cilt 75
Özet
Alkanna Tausch is a small genus which comprises of 34 species and 40 taxa in the flora of Turkey [1]. Biological activity studies of naphthoquinone derivatives obtained from Alkanna species demonstrated antimicrobial, anti-inflammatory, antithrombotic activity and wound-healing properties as well as DNA-topoisomerase I inhibition [2]. As part of our continuing studies to identify antitumor compounds, we herein report for the first time, a cytotoxic activity study of methanol extracts of 17 Alkanna species collected from Turkey. The dried and pulverized roots and aerial parts of plant materials were extracted with methanol, evaporated and freeze-dried. The cytotoxicities of the extracts were evaluated by the MTT assay using six human cancer cell lines (MDA-MB-231, DU145, LNCaP, MCF7, PC-3 and Hep 3B). The extracts obtained from the aerial parts were not active in the dose range of 8–32µG/mL. The root extracts of our endemic species A. cappadocica, A. pseudotinctoria, A. cordifolia, A. tinctoria subsp. subleicarpa, A. tubulosa showed significant and stronger cytotoxic activities compared to the other extracts and positive control CPT-11 (CAMPTOSTAR®, irinotecan) with IC 50 values less than 8µg/mL. Additionally, a bioassay guided fractionation study was performed on A. cordifolia for its inhibitory effects on mammalian DNA topoisomerase I. Some of the fractions obtained during RP (C-18) and silica gel fractionations proved to be very potent topoisomerase I inhibitors at nanogram level (˜12.5ng/ml). Further studies are in progress to identify bioactive molecules. Acknowledgement: This study was supported by Turkish Scientific and Technological Research Council of Turkey (Project No: SBAG-3134). References: [1] Davis, P.H. (1973) „Flora of Turkey and East Aegean Islands“ Vol.3, University Press: Edinburgh. [2] Papageorgiou, V.P. et al. (1999) Angew. Chem. Int. Edit. 38:270–300.