99mTc-exendin-4: Radiolabeling and quality control studies of glucagon-like peptide analog
Makaleler > Tam Makale · 2022
Yazarlar: Derya İLEM ÖZDEMİR, Meliha EKİNCİ, Emre ÖZGENÇ, Evren ATLIHAN GÜNDOĞDU, MAKBULE AŞIKOĞLU
Experimental Biomedical Research, Diğer Kurumların Hakemli Dergileri, Uluslararası, TR Dizin (Ulakbim), 2022, cilt 5, ss 235-243
Özet
Aim: Exendin-4 is a type 2 diabetes antidiabetic drug that is a peptide agonist of the glucagon-like peptide 1 receptor (GLP-1R).Up to date, different exendin-4 compounds have been radiolabeled with many radioisotopes such as 68 Ga, 18 F, 64 Cu and 99m Tc for various purposes like study of over expression of GLP-1R in insulinoma.The purpose of this study is to radiolabel exendin-4 with 99m Tc under appropriate conditions.Methods: In this study, exendin-4 was labeled with 99m Tc, and quality control tests of 99m Tc-exendin-4 were done using ascending radioactive thin layer chromatography (RTLC).Also, the effects of important parameters such as the amount of reducing agent, pH value, incubation time, and radiation dose on the labeling efficiency were investigated.Then, the stability of 99m Tc-exendin-4 was assessed.Results: According to the results, 99m Tc-exendin-4 was prepared with over 95 percent labeling efficiency by a novel, easy, and quick direct method with 30-min incubation time at pH 6.6.To achieve the best radiolabeling condition; 10 µg of exendin-4, 50 µg of stannous chloride (reducing agent) and 37 MBq 99m Tc was used.The RTLC studies indicated that 99m Tc-exendin-4 is stable up to 6 h in room temperature. Conclusion:The obtained results demonstrated that radiolabeled exendin-4 may be a promising agent for GLP-1R imaging studies.Further studies are in progress in order to evaluate receptor binding capacity and biodistribution of the complex in experimental animals.