Response Surface Methodology for Optimization of Food Borne Pathogen Detection in Real Samples Based on Label Free Electrochemical Nucleic Acid Biosensors
Articles > Article · 2011
Authors: Z. Urkut, Pınar KARA, Mehmet Yekta GÖKSUNGUR, M. Ozsoz
Electroanalysis, Journal Indexed in SCI Expanded, International, WoS, 2011, vol 23, pp 2668-2676
Summary
Abstract The response surface methodology (RSM) is used for optimization of foodborne pathogen detection based on label free electrochemical nucleic acid biosensors. Listeria monocytogenes amplicons obtained from food samples are used as model case. The extent of hybridization is determined by using guanine oxidation signals obtained with Differential Pulse Voltammetry and Electrochemical Impedance Spectroscopy. RSM is used to investigate the effects of hybridization parameters including target and salt concentration and hybridization time on the biosensor selectivity. The ratio of electrochemical transductions after hybridization with complementary and noncomplementary targets is the response of the statistical analysis obtaining a detection limit of 267 pM.