Cofactor metals and antioxidant enzymes in cisplatin-treated rats: effect of antioxidant intervention
Articles > Article · 2014
Authors: SUNA SABUNCUOĞLU, Ayşe EKEN, Ahmet AYDIN, Hilal Ozgunes, Hilmi ORHAN
Drug and Chemical Toxicology, Journal Indexed in SCI Expanded, International, WoS, 2014, vol 38, pp 375-382
DOI: 10.3109/01480545.2014.974107
Summary
We explored the association between the activities of antioxidant enzymes and their metallic cofactors in rats treated with cisplatin. The antioxidant effects of aminoguanidine, and a combination of vitamins E and C were investigated. Plasma platin was significantly lower than liver and kidney. Cisplatin treatment caused significant increase in plasma Se-glutathione peroxidase activity. Activities of Se-glutathione peroxidase, glutathione S-transferase, catalase and Cu,Zn-superoxide dismutase have been found to be significantly decreased in liver and kidney compared to controls. Zn levels in these organs were diminished upon cisplatin treatment, while levels of Cu were unaffected. Interestingly, levels of iron, the cofactor of catalase, were found to be significantly increased in liver and kidney. Intervention with aminoguanidine or vitamins was generally prevented cisplatin-caused changes in the activity of enzymes and in the tissue levels of cofactor metals. These observations suggest that relation between activities of enzymes and levels of cofactor metals is multifactorial.