Additional file 1 of Development of EphA2 siRNA-loaded lipid nanoparticles and combination with a small‐molecule histone demethylase inhibitor in prostate cancer cells and tumor spheroids
Makaleler > Tam Makale · 2021
Yazarlar: Oner, Ezgi, Mustafa KOTMAKÇI, Baird, Anne-Marie, Gray, Steven G., Bilge DEBELEÇ BÜTÜNER, Bozkurt, Emir, Ayşe Gülten KANTARCI, Finn, Stephen P.
Figshare, Uluslararası, 2021
DOI: 10.6084/m9.figshare.14183772
Özet
Additional file 1: Fig. S1. Representative Western blot image showing the basal expression levels of EphA2 protein in LNCaP, DU145, and PC-3 prostate cancer cells. Fig. S2. Full scans of Western blot images corresponding to Fig. 6c, d. Marker bands: 250, 150, 100, 75, 50, 37, 25, 20 kDa. Fig. S3. The effect of siEphA2 complexes co-administered with JIB-04 on cell viability in RWPE-1 (A) and PWR-1E (B) normal human prostate epithelial cell lines (48 h). Data are given as mean ± SD of three measurements. Fig. S4. The morphological changes in PC-3 spheroids after co-treatment of siEphA2 complexes with JIB-04. Scale bar, 1000 µm. Fig. S5. Representative phase-contrast images of PC-3 cells showing the changes in scratch area in response to treatments after 0 h, 24 h, 48 h, and 72 h. MRI Wound Healing Tool plugin of ImageJ was used to automatically draw the scratch area. Scale bar, 1000 µm.