SIRT1 siRNA-loaded lipid nanoparticles enhanced doxorubicin-induced cell death in prostate cancer cell lines
Makaleler > Tam Makale · 2021
Yazarlar: Bilge DEBELEÇ BÜTÜNER, Ezgi Oner, Mustafa KOTMAKÇI, Ayşe Gülten KANTARCI
Journal of Drug Delivery Science and Technology, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2021, cilt 66, ss 102670-102670
DOI: 10.1016/j.jddst.2021.102670
Özet
<p id="abspara0010" style="margin-bottom: 16px; padding: 0px; color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;">Most chemotherapeutics induce apoptosis by creating DNA damage in tumor cells. However, high expression of <a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/sirtuin-1" title="Learn more about Sirtuin 1 from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">Sirtuin 1</a><span style="margin: 0px; padding: 0px;"> (SIRT1), which is a <a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/histone-deacetylase" title="Learn more about histone deacetylase from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">histone deacetylase</a> activates DNA repair mechanisms leading to the repair of chemotherapeutic-induced DNA damage and subsequently prevention of apoptosis. Therefore, inhibition of SIRT1 is a useful strategy in cancer therapy to achieve a more efficient eradication of tumor cells.</span></p><p id="abspara0015" style="margin-bottom: 16px; padding: 0px; color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;"><span style="margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px;">In this study, novel <a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/lipoplex" title="Learn more about lipoplexes from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">lipoplexes</a> composed of 1,2-di-O-octadecenyl-3-trimethylammonium </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/propane" title="Learn more about propane from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">propane</a> (DOTMA) containing lipid </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/nanoparticle" title="Learn more about nanoparticles from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">nanoparticles</a> and SIRT1 </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/small-interfering-rna" title="Learn more about siRNA from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">siRNA</a><span style="margin: 0px; padding: 0px;"> have been formulated by the freeze-drying method. SIRT1 silencing effect has been demonstrated by a 2.5- to 4- fold decrease in mRNA and protein expression levels. The efficiency of the lipoplexes to enhance the activity of <a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/doxorubicin" title="Learn more about doxorubicin from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">doxorubicin</a>, a DNA damage-inducing chemotherapeutic agent, has been studied on </span></span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/prostate-cancer" title="Learn more about prostate cancer from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">prostate cancer</a> cell lines with different p53 expression patterns, namely LNCaP, DU145, and PC3. SIRT1 silencing by lipoplexes enhanced DNA damage recognition and increased the activity of doxorubicin on cancer cell death. These results have shown that SIRT1 siRNA-loaded lipid nanoparticles can enhance the efficiency of doxorubicin on prostate cancer cell lines.</p>