Development and radiolabeling of lipid nanoparticles with [99mTc]Tc-HMPAO: Characterization, stability, cytotoxicity and cell binding studies
Makaleler > Tam Makale · 2021
Yazarlar: Evren ATLIHAN GÜNDOĞDU, Meliha EKİNCİ, Emre ÖZGENÇ, Emine Selin Demir, Derya İLEM ÖZDEMİR
Journal of Research in Pharmacy, AHCI İndekslerine Giren Dergi, Uluslararası, Scopus, 2021, cilt 26(1), ss 1105-1118
DOI: 10.29228/jrp.106
Özet
In this study, we aimed to develop new lipid based nanoparticles (LPNs) and radiolabeled LPNs with [ 99m Tc]Tc-HMPAO to investigate its cell binding capacity comparatively with [ 99m Tc]Tc-HMPAO on different cancer cells.According to obtained results, LPNs with zeta potential of -27.4 ± 0.95 mV, particle size of 93.5 ± 1.17 nm, and polydispersity index of 0.35 ± 0.04 were successfully developed.The optimum radiolabeling efficiency was found to be above 90% at 15-min of incubation time.The cell binding capacity of [ 99m Tc]Tc-HMPAO-LPNs was found to be higher than [ 99m Tc]Tc-HMPAO in cancer cell lines.The results demonstrated that [ 99m Tc]Tc-HMPAO-LPNs may be a promising agent for cancer diagnosis alternatively to [ 99m Tc]Tc-HMPAO.