NEW MICROVASCULAR ANASTOMOSIS MODEL WITH AMNIOTIC MEMBRANE
Project no. 57 · Status: Kapanmış · 2016
Summary
With the development of technology, limb amputations have begun to decrease with developing surgical techniques and limb salvage procedures have begun to come to the forefront. This surgeries has progressed parallel to the development of microsurgical techniques. The identification of the microvascular anastomosis technique has been one of the most critical surgical steps of microsurgery. Microvascular anastomosis was first described by Alexis Carrel and the microsurgery experience advancing in the direction of the principles described by him, new suture materials, devices and medicines have led to the identification of new anastomosis methods. The main goals of investigating the new methods are obtaining the highest post-anastomotic vessel patency, repairing the vessel in anatomic fashion with minimum thrombosis, inventing easy and cheaper anastomose methods. For this purpose, an anastomosis model using amniotic membrane was performed as a model that was not performed before. A total of 22 Wistar-Albino rats, 11 control and 11 experimental animals, were used in this study. Under general anesthesia, anastomosis model was applied to rat femoral artery in which amniotic membrane was combined with few stitches. Conventional anastomosis technique was performed to the control group. At the end of the experiment, the effects of the model on the patency and histological structure of the vessels were evaluated. As a result, normal patency was determined radiologically and macroscopically in all anastomoses. No thrombosis or aneurysm was detected in any anastomoses (n:0) In the angiographic study, vessel patency was detected in control and experimental groups. The duration of the anastomosis of the experiment group was completed in a shorter period in a statistically significant manner. In histological studies, anti-e-NOS staining revealed that endothelin was significantly oversprayed in the experimental group. This method describes a new anastomosis model and the results are promising for additional experimental studies and for implementation in the clinic. With additional studies, we believe that this experimental technique can be put into clinical practice as an alternative to the conventional microvascular anastomose technique.