ISOLATION OF SYMBIOTIC NITROGEN-FIXING BACTERIA FROM SOME LEGUMENS AND ANALYSIS OF OF CULTURAL CHARACTERISTICS
Project no. 22147 · Status: Kapanmış · 2020
Summary
Nowadays, researchers on Rhizobial species that perform symbiotic nitrogen fixation in the roots of legumes that are grown for agricultural purposes are being studied intensively. Rhizobia are Gram-negative, soil bacteria which belong to the Alphaproteobacteria class of Protobacteria. Rhizobium species can form a symbiotic relationship with the roots of legumes. This symbiosis is important because plants cannot use nitrogen directly and they need nitrogen-fixing bacteria such as Rhizobia. Because of the Nod genes they have, these bacteria form nodules in the roots and convert nitrogen into ammonia. On the other hand, plants provide Rhizobium with organic compounds formed as a result of photosynthesis. This symbiotic relationship is same for all rhizobial species. In this study, the root nodules of some legumes in Izmir and its surroundings were collected and the isolation of Rhizobium species and their identification with cultural and methods were carried out. The isolation of the bacteria was carried out in YEMA medium. The colony morphologies and the ability to absorb Congo Red dye of 20 isolates were observed in YEMA medium with Congo Red dye. In addition, motility and catalase tests were performed. Molecular identification of isolates was carried out by conventional PCR method. According to the results obtained from the study, in which primers specific for the NodC gene region were used, the NodC gene region was found in 3 isolates out of 20 isolates.