Paper-based sensors prepared with nanofibers and their applications in biomarker analysis

Project no. 31999 · Status: Kapanmış · 2023

Summary

<p>The&nbsp;early&nbsp;detection&nbsp;of&nbsp;biological&nbsp;markers&nbsp;in&nbsp;body&nbsp;fluids&nbsp;plays&nbsp;a&nbsp;vital&nbsp;role&nbsp;in&nbsp;clinical&nbsp;diagnosis&nbsp;for&nbsp;both&nbsp;identifying&nbsp;diseases&nbsp;and&nbsp;predicting&nbsp;potential&nbsp;physiological&nbsp;risks.&nbsp;Point-of-care&nbsp;testing&nbsp;(POCT)&nbsp;technologies&nbsp;enable&nbsp;the&nbsp;rapid&nbsp;and&nbsp;economical&nbsp;analysis&nbsp;of&nbsp;biomarkers,&nbsp;even&nbsp;at&nbsp;low&nbsp;concentrations.&nbsp;With&nbsp;the&nbsp;integration&nbsp;of&nbsp;nanomaterials&nbsp;into&nbsp;these&nbsp;systems,&nbsp;it&nbsp;has&nbsp;become&nbsp;possible&nbsp;to&nbsp;produce&nbsp;test&nbsp;kits&nbsp;that&nbsp;are&nbsp;highly&nbsp;cost-effective,&nbsp;portable,&nbsp;and&nbsp;practical&nbsp;to&nbsp;use.</p><p></p><p>Within&nbsp;the&nbsp;scope&nbsp;of&nbsp;this&nbsp;thesis&nbsp;research,&nbsp;a&nbsp;nanofiber-based&nbsp;colorimetric&nbsp;sensor&nbsp;was&nbsp;developed&nbsp;for&nbsp;the&nbsp;detection&nbsp;of&nbsp;the&nbsp;CD36&nbsp;protein,&nbsp;which&nbsp;is&nbsp;associated&nbsp;with&nbsp;atherosclerosis.&nbsp;For&nbsp;the&nbsp;construction&nbsp;of&nbsp;the&nbsp;sensor&nbsp;platform,&nbsp;bead-free&nbsp;nanofibers&nbsp;possessing&nbsp;a&nbsp;high&nbsp;surface-to-volume&nbsp;ratio&nbsp;were&nbsp;obtained&nbsp;via&nbsp;the&nbsp;electrospinning&nbsp;method&nbsp;using&nbsp;Polycaprolactone&nbsp;(PCL)&nbsp;and&nbsp;polyallylamine&nbsp;hydrochloride&nbsp;(PAH)&nbsp;polymers.&nbsp;To&nbsp;detect&nbsp;CD36,&nbsp;bioconjugates&nbsp;containing&nbsp;protein-specific&nbsp;aptamers&nbsp;and&nbsp;gold&nbsp;nanoparticles&nbsp;(AuNPs)&nbsp;were&nbsp;prepared,&nbsp;incubated&nbsp;with&nbsp;CD36&nbsp;samples&nbsp;of&nbsp;varying&nbsp;concentrations,&nbsp;and&nbsp;applied&nbsp;to&nbsp;the&nbsp;platform.&nbsp;Color&nbsp;changes&nbsp;observed&nbsp;on&nbsp;the&nbsp;platform,&nbsp;which&nbsp;occurred&nbsp;in&nbsp;parallel&nbsp;with&nbsp;the&nbsp;increase&nbsp;in&nbsp;CD36&nbsp;concentration,&nbsp;were&nbsp;recorded&nbsp;and&nbsp;analyzed&nbsp;using&nbsp;a&nbsp;smartphone&nbsp;camera.&nbsp;The&nbsp;developed&nbsp;nanofiber-based&nbsp;sensor&nbsp;exhibited&nbsp;a&nbsp;linear&nbsp;working&nbsp;range&nbsp;of&nbsp;25–500&nbsp;ng/mL&nbsp;and&nbsp;a&nbsp;limit&nbsp;of&nbsp;detection&nbsp;(LOD)&nbsp;of&nbsp;27.78&nbsp;ng/mL.</p>

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