Investigation of the expression level of miR-146a in archival sera used in the diagnosis and follow-up of cystic echinococcosis patients
Project no. 29573 · Status: Kapanmış (Yayın bekleniyor) · 2023
Summary
<p>Introduction: Cystic Echinococcosis (CE) is a zoonotic disease characterized by fluid-filled cysts in internal organs and is an important public health problem. There are still no standardized diagnostic methods ort he diagnosis of CE. In this study, we aimed to investigate the presence of miR-146a in the archived serum samples of CE patients ort he first time and to evaluate its role in the diagnosis and follow-up of CE over a three-year period. </p><p>Method: Archived serum samples of 39 CE patients, 56 follow-up samples of 14 CE patients and 3 healthy control samples were included in the study. Mir16a levels were evaluated in these archived serum samples using quantitative real-time PCR (qRT-PCR).</p><p>Results: The miR146 gene was shown to be significantly up-regulated in the archived serum samples of CE patients compared to the archived samples of the healthy control group (p < 0.05). In addition, it was observed that the expression level of the miR146 gene in the serum samples of follow-up patients decreased significantly in the samples taken in the third year after surgery compared to the follow-up blood samples taken in the previous years.</p><p>Conclusions:. These findings suggest that miR-146a may have potential as a diagnostic biomarker for CE and highlight the need for further research with a larger patient panel to confirm its potential.</p>