PHYSICAL, CHEMICAL AND BIOLOGICAL CHARACTERIZATION OF IMPLANT SURFACES COATED WITH BIOACTIVA PHA, PHB AND PHBV
Project no. 614 · Status: Kapanmış · 2015
Summary
The aim of this work is to develop an antibiotic coating on the surface of a titanium grade 4 plate to determine its antibacterial and surface properties. A promising approach for reducing the occurrences of infections is to provide the surfaces of biomedical devices and dental implants with features that are unfavourable for bacterial attachment and proliferation. Therefore to combine the antibacterial properties of antibiotic-loaded Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with titanium, as the base material for implants, in order to obtain biomimetic surfaces with antibacterial activity. The titanium surfaces were linked to both PHBV and hydroxyapatite using electrospinning technique. This attachment was carried out by firstly activating the titanium surfaces with sodium hydroxide. Further functionalization of the activated surfaces were carried out with antibiotics which are ciprofloxacin, vancomycin hydrochloride and lincomycin hydrochloride coated on the titanium surface. The success of the surfaces functionalization as well as the different superficial linkages was confirmed by means of the following characterization methods: contact angle, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDAX) Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), Raman spectroscopy. The antibacterial properties of the titanium treated surfaces were tested by means of an in vitro antibacterial assay using a Gram + and Gram – bacteria. Finally drug release kinetic models were detected.