Human Papillomavirus Prevalence and Genotypes in Head and Neck Cancers

Project no. 32534 · Status: Kapanmış (Yayın bekleniyor) · 2024

Summary

<p><strong style="color: rgb(0, 0, 0);">Objective:&nbsp;</strong><span style="color: rgb(0, 0, 0);">To&nbsp;determine&nbsp;the&nbsp;prevalence&nbsp;and&nbsp;genotype&nbsp;distribution&nbsp;of&nbsp;human&nbsp;papillomavirus&nbsp;(HPV)&nbsp;in</span></p><p><span style="color: rgb(0, 0, 0);">head&nbsp;and&nbsp;neck&nbsp;squamous&nbsp;cell&nbsp;carcinomas&nbsp;(HNSCC)&nbsp;and&nbsp;to&nbsp;evaluate&nbsp;the&nbsp;associations&nbsp;between</span></p><p><span style="color: rgb(0, 0, 0);">HPV&nbsp;positivity,&nbsp;clinicopathological&nbsp;characteristics,&nbsp;p16&nbsp;expression,&nbsp;and&nbsp;p53&nbsp;pattern.</span></p><p><strong style="color: rgb(0, 0, 0);">Materials&nbsp;and&nbsp;Methods:&nbsp;</strong><span style="color: rgb(0, 0, 0);">This&nbsp;prospective&nbsp;observational&nbsp;study&nbsp;was&nbsp;conducted&nbsp;at&nbsp;Ege&nbsp;University&nbsp;Faculty&nbsp;of&nbsp;Medicine&nbsp;between&nbsp;October&nbsp;1,&nbsp;2024,&nbsp;and&nbsp;April&nbsp;15,&nbsp;2026.&nbsp;A&nbsp;total&nbsp;of&nbsp;160&nbsp;patients&nbsp;who&nbsp;underwent&nbsp;surgery&nbsp;for&nbsp;suspected&nbsp;HNSCC&nbsp;were&nbsp;included.&nbsp;HPV&nbsp;DNA&nbsp;was&nbsp;analyzed&nbsp;in&nbsp;tumor&nbsp;tissue&nbsp;samples&nbsp;using&nbsp;polymerase&nbsp;chain&nbsp;reaction&nbsp;(PCR),&nbsp;and&nbsp;genotyping&nbsp;was&nbsp;performed&nbsp;in&nbsp;HPV-positive&nbsp;cases.</span></p><p><span style="color: rgb(0, 0, 0);">Demographic&nbsp;and&nbsp;clinicopathological&nbsp;data&nbsp;were&nbsp;recorded.&nbsp;p16&nbsp;expression&nbsp;and&nbsp;p53&nbsp;pattern</span></p><p><span style="color: rgb(0, 0, 0);">were&nbsp;evaluated&nbsp;by&nbsp;immunohistochemistry.&nbsp;Overall&nbsp;survival&nbsp;was&nbsp;analyzed&nbsp;using&nbsp;the&nbsp;Kaplan–</span></p><p><span style="color: rgb(0, 0, 0);">Meier&nbsp;method.</span></p><p><strong style="color: rgb(0, 0, 0);">Results:&nbsp;</strong><span style="color: rgb(0, 0, 0);">HPV&nbsp;DNA&nbsp;positivity&nbsp;was&nbsp;detected&nbsp;in&nbsp;8&nbsp;patients&nbsp;(5%).&nbsp;Among&nbsp;HPV-positive&nbsp;cases,&nbsp;HPV16</span></p><p><span style="color: rgb(0, 0, 0);">was&nbsp;identified&nbsp;in&nbsp;50%,&nbsp;HPV18&nbsp;in&nbsp;25%,&nbsp;and&nbsp;HPV82&nbsp;and&nbsp;HPV6&nbsp;in&nbsp;the&nbsp;remaining&nbsp;cases.&nbsp;HPV</span></p><p><span style="color: rgb(0, 0, 0);">positivity&nbsp;was&nbsp;significantly&nbsp;higher&nbsp;in&nbsp;laryngeal&nbsp;tumors&nbsp;than&nbsp;in&nbsp;oral&nbsp;cavity&nbsp;tumors&nbsp;(p=0.027).</span></p><p><span style="color: rgb(0, 0, 0);">Smoking&nbsp;was&nbsp;more&nbsp;frequent&nbsp;in&nbsp;HPV-negative&nbsp;patients&nbsp;(p=0.013).&nbsp;p16&nbsp;expression&nbsp;was&nbsp;more</span></p><p><span style="color: rgb(0, 0, 0);">common&nbsp;in&nbsp;HPV-positive&nbsp;tumors&nbsp;(p&lt;0.001),&nbsp;and&nbsp;wild-type&nbsp;p53&nbsp;pattern&nbsp;was&nbsp;associated&nbsp;with</span></p><p><span style="color: rgb(0, 0, 0);">HPV&nbsp;positivity&nbsp;(p=0.011).&nbsp;The&nbsp;sensitivity&nbsp;and&nbsp;specificity&nbsp;of&nbsp;p16&nbsp;expression&nbsp;for&nbsp;predicting&nbsp;HPV</span></p><p><span style="color: rgb(0, 0, 0);">presence&nbsp;were&nbsp;62.5%&nbsp;and&nbsp;90.8%,&nbsp;respectively.&nbsp;No&nbsp;significant&nbsp;association&nbsp;was&nbsp;observed</span></p><p><span style="color: rgb(0, 0, 0);">between&nbsp;HPV&nbsp;positivity&nbsp;and&nbsp;overall&nbsp;survival&nbsp;(p&gt;0.05).&nbsp;Absence&nbsp;of&nbsp;p16&nbsp;expression,&nbsp;mutant</span></p><p><span style="color: rgb(0, 0, 0);">type&nbsp;p53&nbsp;pattern,&nbsp;immunosuppression,&nbsp;and&nbsp;chronic&nbsp;disease&nbsp;were&nbsp;significantly&nbsp;associated&nbsp;with</span></p><p><span style="color: rgb(0, 0, 0);">mortality&nbsp;(p=0.015,&nbsp;p=0.027,&nbsp;p=0.035,&nbsp;and&nbsp;p=0.001,&nbsp;respectively).</span></p><p><strong style="color: rgb(0, 0, 0);">Conclusion:&nbsp;</strong><span style="color: rgb(0, 0, 0);">Although&nbsp;HPV&nbsp;prevalence&nbsp;was&nbsp;low,&nbsp;HPV&nbsp;positivity&nbsp;was&nbsp;significantly&nbsp;associated&nbsp;with&nbsp;p16</span></p><p><span style="color: rgb(0, 0, 0);">expression&nbsp;and&nbsp;wild-type&nbsp;p53&nbsp;pattern.&nbsp;Combined&nbsp;evaluation&nbsp;of&nbsp;p16&nbsp;and&nbsp;p53&nbsp;patterns&nbsp;may</span></p><p><span style="color: rgb(0, 0, 0);">contribute&nbsp;to&nbsp;predicting&nbsp;HPV&nbsp;DNA-positive&nbsp;tumors.</span></p><p></p>

View details