Effect of breeder age, storage duration, and preincubation on egg yolk utilization and nutrient transporters in intestine
Project no. 22803 · Status: Kapanmış (Yayın bekleniyor) · 2021
Summary
<p>This project aimed to examine the effect of breeder age and the preheating of eggs stored for long-period on development and expression of nutrient transporters in the intestine of chicks. A total of 880 hatching eggs obtained from young (31 weeks old) and old (52 weeks old) Ross breeders were used. Eggs from both age groups were divided into 4 groups. While the 1st group was stored for 3 days (3), the 2nd group was stored for 12 days (12), the 3rd group was preheated at 37.5 °C for 6 hours before 12 days of storage (12+BPH), and the 4th group was preheated on d 4 and 8 at 37.4C for 3 hours during 12 days of storage (12+DPH). In eggs stored for a long time with both preheating methods, blastoderm development was preserved and reached a more advanced level. Storage conditions did not affect chick weight. The interaction between breeder age and storage conditions was found to be significant for yolk sac protein and fat content of chicks. The length and width of the villus increased in the chicks developed in the 12+DPH eggs of old breeders. SGLT1 expression in the jejunum was not affected by breeder age and storage conditions. Compared to the young breeders, the expression of PEPT1 increased in the chicks developed in the 12+BPH eggs of the old breeders, while the expression of GLUT5 decreased in the 12+DPH chicks. Although the change in PEPT1 was not significant in young breeders with 12 days of egg storage compared to those stored for 3 days, GLUT5 expression of chicks increased in chicks from eggs stored for 12 days either preheating or not. These findings suggest that breeder age should be considered before preheating the eggs for long-period storage.</p>