The effects of monocarboxylate transporter 1–T1470A polymorphism on blood lactate accumulation and lactate elimination rate in male athletes
Project no. 686 · Status: Kapanmış · 2018
Summary
Introduction and Aim: Elimination of lactate from cells is facilitated via monocarboxylate transporters (MCTs). A polymorphism in MCTs can effect lactate elimination. The effects of MCT1-T1470A polymorphism (MCT1P) on blood lactate accumulation (LA) and lactate elimination rate (LER) are unclear in anaerobic sportsmen. The purpose of this study is to investigate the effects of MCT1P on blood LA and LER in male athletes. Methods: Blood samples were taken from 42 anaerobically trained athletes (ages; 21,78±2,67) and 39 sedantary males (ages; 23,02±3,28). Hemograms were determined by venous blood samples. Yoyo intermittent recovery test (level 1) was used for endurance performance. 5(LA5), 15(LA15) and 30(LA30) minutes after the test, blood samples were taken from finger tips to determine LA (by YSI 1500 lactate analyzer). To obtain LER15 and LER30, the difference between the LA values of 5th min with 15th min and 30 min was divided by 10, and 25 to obtain LER15 and LER30, respectively. After polymerase chain reaction-analysis conducted by the genomic DNA samples from the peripheral blood; MCT1P was determined by next generation sequencing analysis. Results: There was no significant difference between athletes and their controls for LA and for LER15 and LER30 (p> 0.05). There was no significant difference for LA and LERs between T carrier (TT+TA) and AA homozygous (p>0.05). No role of this polymorphism on these results was observed. Conclusions: These findings suggest that MCT1P has no significant role on lactate accumulation and lactate elimination rate after a maximal exercise test in anaerobically trained male athletes. Keywords: monocarboxylate transporters, lactate elimination, MCT1 T1470A polymorphism