BIOINFORMATIC AND EXPRESSION ANALYSIS OF MULTI DRUG RESISTANCE - RELATED ABC TRANSPORTER GENES (MDR) IN OYSTER MUSHROOM (PleurotusOsteratus) GENOME
Project no. 20023 · Status: Kapanmış · 2018
Summary
ATP Binding Casette proteins which are one of the structures that control many metabolic pathways at cell membrane and constitutes one of the greatest protein families, take role at substance transportation by using ATP energy. ABC proteins provide the required energy to carry out its biological function by hydrolyzing ATP. Moreover, a major part of ABC proteins which are also known as ABC transporters, take role in transportation of a variety of substrates through biological membranes. Soluble ABC proteins are not involved in transmembrane transportation, however they take an important role in cellular processes such as ribosomal biogenesis and translation of mRNA. Multidrug Resistance (MDR) subfamily which is one of the subfamily of ABC proteins, take role in antimicrobial peptides and lipid transport, mating factors (pheromones) transport, mitochondrial porphyrin uptake, multidrug resistance, eukaryotic peptide export, processing of antigens, protection from the oxidative stress, heavy metals tolerance, etc. In this study, eight genes encoding PoMDR proteins were identified, and the phylogenetic tree of the genes encoding PoMDR proteins was constructed by the bioinformatics analysis of oyster mushroom (Pleurotus osteradus) genome. Besides, expression level of PoMDR genes at stalk and cap were identified for the first time. According to the results of this study, it was concluded that PoMDR2, PoMDR3, PoMDR7 and PoMDR8 genes have parallel expression, and that they can also be involved in the biosynthesis and transport of fibric proteins to the required units. On the other hand, it was concluded that PoMDR1, PoMDR5 and PoMDR7 could be involved in pheromone transport in nucleus-associated signal metabolism. The results also suggest that most of the genes identified were involved in the transport of vitamin B12 in particular.