Investigation of genes related to salt stress tolerance by screening cDNA library in the extreme halophyte Eutrema parvulum plant.
Project no. 21958 · Status: Kapanmış (Yayın bekleniyor) · 2020
Summary
<p>Salinity is one of the environmental factors that negatively affects plant growth and productivity. Due to their quiescent nature, plants have developed various mechanisms to sense salt stress and provide the necessary responses. Since glycophyte plants, which include most agricultural plants, are not tolerant to high soil salinity, decreases in plant production are inevitably observed in saline soils. Halophyte plants growing near the seashore or in areas with other terrestrial saline soils have adapted to soil salinity. These plants have managed to minimize the negative effects of soil salinity through different adaptations at the molecular, biochemical and physiological levels, as well as at the anatomical level. In recent years, the extreme halophytic plant named Eutrema parvulum has been used as a new model plant in salt stress tolerance studies due to its features such as its highly similar genome to the model plant Arabidopsis (~90% sequence similarity) and its short life cycle. E. parvulum grows naturally around the Salt Lake in our country and can survive in 600 mM NaCl, which is roughly equal to the salinity of sea water. The aim of this research project is to create a cDNA library from E. parvulum plants subjected to salt stress, to express the obtained cDNA library in yeast (Saccharomyces cerevisiae) and to identify colonies carrying genes that provide tolerance to salt stress, to sequence and identify the genes from the obtained colonies and to express the identified genes in E. parvulum plants. Their expression under salt stress is examined by qRT-PCR. As a result of the project, it is aimed to identify new genes involved in salt stress tolerance in salt plants.</p>