Investigation of Laccase Production Using Some Industrial and Agricultural Wastes
Project no. 23522 · Status: Kapanmış (Yayın bekleniyor) · 2022
Summary
<p>Agricultural and industrial wastes offer a valuable source of bioactive compounds, making the use of these wastes an increasingly important area of research for the production of valuable materials. Along with effective waste management practices, it is essential to develop cost-effective processes to produce valuable compounds. Therefore, the aim of this study was to increase the efficiency of laccase production using a newly identified strain, <em>Trametes gibbosa</em> KSY9, by using these waste materials. In this study, which is important in terms of local and national enzyme production, wheat bran, sawdust and cone waste were determined as the selected substrates. In 250ml flasks, 5g wheat bran, sawdust and pine cones were added. Molasses and dyed wastewater were used as the moistening fluid. The substrates were moistened to 70%. A total of 12 different combinations of selected industrial wastes and moistening fluids were made. <em>T. gibbosa</em> was removed from the petri dish in which it was grown, as inoculation, and inoculated into the flasks. The erlenmayer flasks were statically incubated at 30°C for 14 days. The changes in the substrates used in the study before and after inoculation were demonstrated by scanning electron microscopy. The highest laccase activity was obtained as 115866.7 U/L on the 15th day in the erlenmayer flasks where wheat bran and dyed wastewater were used. A 4.27-fold increase was achieved compared to the control. In one of the mixture combinations (dyed wastewater, cones, sawdust and wheat bran), the enzyme activity was found to be 39266.7 U/L on the 10th day.</p><p>These results showed that it is possible to obtain high value-added products such as laccase, which have application areas in different fields, as well as eliminating both industrial and agricultural wastes and facilitating their recycling cycles. It would be appropriate to determine the sustainable aspects of the systems created with scale-up projects.</p>