Comparison of the Reprogramming Effect of Embryonic and Fetal Body Fluids on Prostate Cancer
Project no. 32424 · Status: Kapanmış (Yayın bekleniyor) · 2024
Summary
<p> During the embryonic period, cell differentiation, proliferation, and migration occur in an effective and continuous manner, regulated throughout pregnancy, with numerous signaling pathways actively involved in this process. In recent years, the relationship between the molecular mechanisms of cancer and embryonic development has attracted increasing attention. The reactivation of mechanisms potentially relevant to cancer therapy through molecules or pathways associated with the embryonic period is of particular interest to researchers in this field. In the planned project, the potential inhibitory effects of body fluids associated with the embryonic and fetal periods during pregnancy on prostate cancer will be analyzed. The Du-145 prostate cancer cell line will be used, and this cell line will be cultured in conditioned media obtained from the primary culture of body fluids related to the embryonic and fetal periods during pregnancy, including amniotic fluid, amniocentesis fluid, and chorionic villus fluid. Following gene expression analysis and immunofluorescence staining, the proportions of these cell lines will be compared between the control and experimental groups.</p>