Molecular Identification of Genes Responsible for Biodegradation of Paratoluic Acid, Terephthalic Acid and 4-Carboxybenzaldehyde Chemicals and Determination of Pathways
Project no. 20395 · Status: Kapanmış · 2019
Summary
Various chemicals, especially paratoluic acid, terephthalic acid and 4-carboxybenzaldehyde, which constitute the raw material of materials such as plastics, textile fibers, paints and adhesives that we use today, are mixed with wastewater during production. As a result of the increase in the industry, the chemicals in the wastewater, whose production is increased and not treated, are released into the environment and have a negative impact on human health. Therefore, it is degrade to treat such toxic chemicals. Today, the biological treatment preferred for the treatment of chemicals; It is an economical, useful and environmentally friendly method compared to chemical and physical treatment. In biological treatment, the biodegradation of harmful chemicals takes place through microorganisms. Today, there are companies where bacteria are used as commercial products in many fields such as wastewater, oil and odor removal, and bacteria or enzymes produced are available in the market as biotechnological products. A consortium of 16 bacteria, known to degrade pTol, TPA and 4-cba chemicals within 24 hours, was used in this study. For each of these bacteria forming the consortium, pTol, 4-cba and TA chemical degradation efficiency was determined by HPLC (High Performance Liquid Chromatography). At the same time, primers specific to gene regions of bacteria that allow them to degrade chemicals were synthesized and the presence of primer-specific gene regions of organisms that break down pTol, TPA and 4-cba chemicals were determined. It has been determined whether the presence of these genes originates from plasmid or genomic DNA. It will be a study that will shed light on determining gene regions of bacteria, transferring different gene regions to plasmid in future studies and obtaining recombinant DNA and studying gene expression levels.