Investigation of the APG-IV-dependent evolutionary process of esterase and peroxidase activities in Pollen-Stigma interaction
Project no. 28463 · Status: Kapanmış · 2023
Summary
<p>The objective of this research is to investigate the presence of ROS and esterases in primitive and advanced groups of angiosperms, as defined by the Angiosperm Phylogeny Group IV (APG-IV) system. This will enable a better understanding of plant reproductive systems by revealing the relationship between ROS and esterases and plant evolution in the pollen-stigma interaction. In order to understand this relationship correctly, the study tested esterase, dehydrogenase, and peroxidase activities, as well as ROS/H2O2 accumulations on the stigmas of species selected from different stages according to APG-IV. Additionally, the germination status of pollen on the stigma was checked. The data obtained were interpreted according to APG-IV, and the tests were evaluated from an evolutionary perspective. Stigma receptivity of all studied species was determined depending on flower development stages. At the conclusion of the study, it was established that NADPH oxidase activity, responsible for ROS production, occurs abruptly, earlier, and more intensely not only in basal angiosperms but also in monocot groups. The accumulation of H2O2, one of the ROS groups, generally intensifies after the anthesis of the flower in all groups. Esterase activity was observed to vary across all groups. Furthermore, esterase, dehydrogenase, peroxidase, and ROS/H2O2 accumulations in eudicot groups were found to be more stable than those observed in other groups.</p>