Investigation of the effects of ARHGAP42 gene polymorphism on blood pressure and arterial stiffness in patients with hypertension
Project no. 20208 · Status: Kapanmış (Yayın bekleniyor) · 2018
Summary
Hypertension is a systemic disease characterized by high blood pressure, and it is an important health problem because it is common in the community and causes serious complications. Genetic and environmental factors are effective in the development of the disease. Recently, the association of the Rho GTPase activating protein 42 (ARHGAP42) genotype with the development of hypertension has been demonstrated. In this study, the relationship of rs604723 (C/T) gene polymorphism, located in the first intron of ARHGAP42, with hypertension-related blood pressure, ambulatory blood pressure and arterial stiffness parameters was investigated. For the study, peripheral blood samples were taken from 63 patients with a diagnosis of hypertension, and ARHGAP42 rs604723 gene polymorphism was determined following DNA isolation and PCR method. Blood pressure, ambulatory blood pressure and arterial stiffness measurement values were recorded in addition to the demographic data of the patients with hypertension in the study group. The control group consisted of a total of 100 healthy subjects. When the study and control group subjects were compared with each other, heterozygous polymorphic CT (~2-fold) and homozygous polymorphic TT (1.58-fold) genotypes were found to be higher in study group subjects, and the increase in CT genotype was statistically significant (p = 0.006). Similarly, the frequency of polymorphic T allele was found to be high (~1.89-fold) and statistically significant (p = 0.003) in the study group cases. In addition, heterozygous polymorphic CT and homozygous polymorphic TT genotypes were found to be statistically significant with carotid-femoral pulse velocity, which is a measure of arterial stiffness, and the number of anti-hypertensive drugs used (p = 0.025 and p = 0.057, respectively). In conclusion, ARHGAP42 rs604723 (C/T) gene polymorphism was found to be associated with arterial stiffness and anti-hypertensive drug use in patients with hypertension, but not with blood pressure and ambulatory blood pressure values. In the continuation of this study, it was planned to investigate the relationship between the genotypes of the cases and the anti-hypertensive drugs they were used. Thus, in the future, it will be possible to select the appropriate drug or drugs according to the genotypes and to carry out personalized treatments by looking at the rs604723 gene polymorphism of patients with hypertension.