Investigation of the Effects on Some Human Pathogens of Antimicrobial Peptides in Frog Species Included in Rana genus (R. dalmatina, R. macrocnemis, R. holtzi, R. tavasensis) Living in Our Country
Project no. 21881 · Status: Kapanmış · 2020
Summary
<p>Frog skin secretion is a very rich source of antimicrobial peptides (AMPs). AMPs are molecules that form the first line of defense against pathogens that enter the body through the skin in frogs and are important elements of natural immunity. AMPs are known as species-specific peptides that differ in different families, genera, species and even subspecies. These peptides, synthesized in ribosomes, are ≤5 kDa and have broad-spectrum microbicidal activity. The physical nature of the mechanisms possessed by AMPs prevents the microorganism from developing resistance to this peptide. These peptides are therefore considered potentially therapeutic agents. Striking variations in the expression of AMPs in frog skin can be used to discover new molecular and structural motifs that target specific microorganisms for which therapeutic defenses are scarce.</p><p>In this study, protein profiles in the skin secretions of <em>Rana dalmatina, Rana macrocnemis, Rana tavasensis </em>and <em>Rana holtzi</em>, which belong to the rana genus widespread in our country, were determined by Tricine Sodium dodecyl sulfate polyacrylamide gel (SDS PAGE) electrophoresis. Additionally, inhibition zones were measured using the plate pit method on <em>Esherica coli, Staphylococcus aureus, Salmonella typhimurium, Listeria monocytogenes</em> and <em>Bacillus cereus</em> bacteria. According to the Trisine SDS Page electrophoresis results, one peptide under 5 kDa was found in the secretions of <em>R. dalamatina, R. macrocnemis</em>, and <em>R. tavasensis</em>, and two in the secretions of <em>R. holtzi</em>. According to the plate well activity results, it was determined that the secretions of no species showed activity on <em>E. coli</em>, while all species showed activity on <em>B. cerus.</em> It was determined that the skin secretion of <em>R. dalmatina</em> showed the most activity against bacteria, while the skin secretion of <em>R. holtzi </em>showed the least activity.</p>