THE ROLE OF DUAL SPECIFIC PHOSPHATASE 29 (DUSP29/ DUPD1) AS A POTENTIAL TREATMENT TARGET IN THE NATURAL RESISTANCE OF SKELETAL MUSCLE TISSUE AGAINST CANCER METASTASIS
Project no. 23742 · Status: Kapanmış (Yayın bekleniyor) · 2022
Summary
<p>One of the most prominent features of cancer is its ability to metastasize; metastasis is the primary cause of cancer-related deaths. Despite this, metastasis to muscle tissue is rarely observed. In this study, we aimed to test whether the resistance of muscle tissue to metastasis is due to the high expression of the serine-threonine phosphatase DUPD1/DUSP27 in muscle tissue. We cultured B16-F10 melanoma and TN breast cancer cell lines with myoblast cell lines to examine the myotube transformation of melanoma cells and the apoptotic/antiproliferative effects of cell-free myoblast media on tumor cells. To test the hypothesis that DUPD1/DUSP29 plays a role in the natural resistance of muscle tissue to metastasis, we inhibited DUPD1/DUSP29 using siRNA and observed how the inhibition of the gene affected phenotypic changes. This project found that the effect of DUPD1/DUSP29 in preventing cancer metastasis was less significant than expected in the study. To fully understand the effects of these genes, more comprehensive studies involving different cancer types, cell types, and experimental conditions are necessary. Such detailed and extensive studies can help better understand the functions of the gene and its potential importance as a therapeutic target. These results suggest that the hypothesis should be tested again with different cell lines, varying siRNA efficiencies, or alternative gene silencing methods.</p>