Evaluation of the Dynein Heavy Chain 5 and Creatine Kinase Levels in Cervical Fluid and Plasma for Early Diagnosing of Ectopic Pregnancy
Project no. 44 · Status: Kapanmış · 2016
Summary
Ectopic pregnancy is a life threatening problems in reproductive ages. Diagnosing of ectopic pregnancy in early period provides to reducing mortality and morbidity and gives an opportunity for medical treatment to preserve fallopian tubes. Evaluation of cervical fluid for determining of ectopic pregnancy with new promising markers provided different aspect for diagnosing of ectopic pregnancy in current study.In this prospective clinical study, ectopic pregnant patients as ectopic pregnancy group (n=46), intrauterine pregnant patients as ectopic pregnancy group (n=29) and not-pregnant patients as non-pregnancy group (n=10) participated to study. Cervical fluid samples were collected with using merocel sponge. Additionally, serum samples were obtained from patients. DNAH5 and CK levels were determined by enzyme linked immunosorbent assay kits in samples. Reduced cervical fluid DNAH5 levels was diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 3.42 ng/mL; 25%–75% percentile 0 - 9.56 ng/mL vs median 6.14 ng/mL; 1.40 - 8.31 ng/mL; p<0.001). On the other hand, DNAH5 protein was not detected in non-pregnant patients’ samples. Additionally, statistical significant increased cervical fluid CK levels were diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 4477.61 IU/L; 0 – 64925.37 IU/L vs 0 IU/L; 0 – 6832.30 IU/L; p=0.006). As a result, measuring of CK and DNAH5 in cervical fluid could be promising markers for early diagnosing of ectopic pregnancy. Decreased DNAH5 levels in cervical fluid might be result from abnormal cilia function in ectopic pregnant patients.