Investigation of nasopharyngeal bacterial microbiota in allergic rhinitis and asthma patients by next generation sequencing
Project no. 20225 · Status: Kapanmış (Yayın bekleniyor) · 2018
Summary
Asthma has been the most common childhood disease affecting approximately one in ten children in the world in recent years. In addition, this disease affects the normal daily life of children and leads to an increase in emergency service admissions. The underlying mechanism of asthma is a complex result of genetic and environmental factors, and it is not known whether there is a causal relationship between airway microbiota and asthma. In this study, it was aimed to compare the nasopharyngeal bacterial microbiota of healthy children with asthma and allergic rhinitis and to determine possible microbial dysbiosis in patients with asthma and allergic rhinitis. A total of 15 patients, 7male and 8 female, with a median age of 12 (7-17 years), were included in the study. 16S metagenomics and upper respiratory tract microbiota were determined from nasal lavage samples of 15 patients, including 5 asthma, 5 allergic rhinitis, and 5 healthy controls, included in the study. The most dominant phylum in the upper respiratory tract microbiota in asthma patients was Firmucutes, while Proteobacteria were found in the healthy control group and allergic rhinitis group. The six most dominant genera in the upper respiratory tract microbiota of asthma patients were identified as Dolosigranulum, Corynebacterium, Haemophilus, Staphylococcus, Streptococcus and Moraxella, respectively. In the upper respiratory tract microbiota of the healthy control group, the most dominant genera were Moraxella, Dolosigranulum, Haemophilus, Corynebacterium, Staphylococcus and Streptococcus, respectively. It was determined that the ratio of Moraxella genus decreased and the ratios of Staphylococcus, Streptococcus and Corynebacterium species increased in asthma patients compared to the control group. In addition, it was found that the relative ratio of Dolosigranulum and Corynebacterium species in the upper respiratory tract microbiota increased in children born with normal birth and brestfeeding for a long time. When the findings obtained in the study and the literature findings were evaluated together, a definite profile that could distinguish between asthma and healthy microbiota was not determined. In conclusion, in order to determine how upper respiratory tract microbiota affects asthma pathophysiology, well-planned longitudinal studies that examine the immunogenetic properties and epithelial responses of the cases are needed.