Evaluation of In Vitro Anti-Prostate Cancer Effects of Camptothecin-Loaded Mesoporous Silica Nanoparticles
Project no. 29643 · Status: Kapanmış (Yayın bekleniyor) · 2023
Summary
<p>Cancer treatment has always been the focus of biomedical research and applications worldwide. Surgery, chemotherapy and radiotherapy are the three main methods of treating cancer in the traditional way. However, these therapies are potentially toxic and can cause side effects and multidrug resistance. According to the different nature of therapies, types of cancer treatment can be categorized as targeted therapy, immunotherapy, cancer stem cell therapy, cancer mechanisms and metastases, cancer drug resistance. Nanotechnological materials are used to increase the effectiveness of therapy methods and to carry them. Specifically targeting cell lines is provided, in this context, it is aimed to reduce toxicity while increasing the therapeutic effect. In addition, therapeutic agents are developed by using molecules that will directly affect the cancer mechanism.</p><p>Camptothecin (CPT) used in this project shows its anticancer effect by acting on DNA Topoisomerase I enzyme.</p><p>In this study, Topoisomerase I inhibitor CPT was encapsulated in Mesoporous Silica Nanoparticles (MSN) with indocyanine green used as fluorescent dye and then conjugated with Prostate Specific Antigen (PSMA) for specification to prostate cancer cells.</p><p>Characterization and optimization studies of nanoconjugates were carried out by Transmission Electron Microscopy, Scanning Electron Microscopy, Infrared Infrared Spectroscopy, Dynamic Light Scattering analysis.</p><p>Camptothecin was loaded into MSN and then conjugated with PSMA and targeted to anrogen positive cells. The loading capacity of CPT on nanoparticles was calculated as 96% by High Performance Liquid Chromatography technique. In vitro applications were performed in PC-3, LNCaP and RWPE-1 androgen positive and negative cancer cell lines. As a result of the cytotoxicity study, the anti-proliferative effect of the samples was examined. The samples conjugated with PSMA showed higher cytotoxicity in androgen positive cells and caused cell death. This evaluation was also confirmed by apoptosis.</p><p>With this study, encapsulation and targeting of Camptothecin, a topoisomerase I inhibitor class, will allow the anticancer effect to be realized at low doses and with less cell damage. It is important because it will contribute to the fields of medicine, biotechnology and pharmacology with the possibility of imaging with fluorescent labeling.</p>