Investigation of Cytotoxic Effects of Implant Abutments Manufactured From Different Materials on Fibroblast Cells
Project no. 21669 · Status: Kapanmış (Yayın bekleniyor) · 2021
Summary
<p>Implant treatment is widely used in cases of missing teeth in dentistry. The implant is surgically applied to the edentulous areas of the lower or upper jaw bones. Thus the implant material comes into contact with the soft and hard tissue in the operation area. There are treatment protocols in which the implant abutment is applied immediately after the operation, along with the placement of the implant. When this protocol is applied, the implant abutment material is in direct contact with the soft tissue wound surface in the operation area. Therefore, the use of a biocompatible material should be essential.</p><p>Biocompatibility at the implant- implant abutment – soft tissue interface is very important for a successful implant treatment. Biocompatibility is the ability of a material to produce an appropriate biological response in the area where it is applied. With the technological developments, many implant abutment materials with different structures and different surface properties have taken place in dentistry. The selection of the implant abutment material should be based on the selection of the material that will interact in harmony with the biological environment surrounding the implant abutment.</p><p>In this study; Lithium disilicate reinforced glass ceramic (LiSiO2), polyetheretherketone (PEEK), polymethylmethacrylate (PMMA), titanium alloy (Ti- 6Al- 4V) and zirconia (ZrO2) abutment materials used with the CAD-CAM principle were used. Mouse fibroblast cell line L929 was used in vitro with samples prepared from the substrate materials. Cytotoxicity analysis, apoptosis analysis, interleukin 6 (IL6) and transforming growth factor-ß (TGF-ß) analysis and reactive oxygen species (ROS) analysis with media taken on the 1st, 3rd and 7th days with 24 hours and 48 hours incubation times were performed. Obtained results were evaluated statistically. According to the results of the analysis, no significant difference was found in the abutment material groups in terms of material and day. In the cytotoxicity analysis results, the glass ceramic reinforced with lithium disilicate abutment material contains the values of the control group within the confidence interval in all day and hour evaluations. It is safe to use as a permanent abutment material. PEEK abutment material cytotoxicity analysis results show that its confidence interval does not include the control group values, except for the 3rd day 24 hour value. When PMMA is prepared in a laboratory or clinical setting, the residual monomer released during polymerization is cytotoxic to living cells. The production of abutment materials with the CAD-CAM principle requires that they be produced in the factory. Fabrication and pressing of PMMA eliminates residual monomer formation. Therefore, PMMA abutment material is suitable for use as a long-term temporary material. Titanium and zirconia abutment materials were considered safe according to the analysis results.</p><p>None of the abutment materials can be evaluated as cytotoxic to fibroblast cells; shows similar apoptotic-necrotic values with the control group; IL6 is considered safe in terms of TGF-ß release and ROS activity.</p><p>Keywords; Dental implants; abutment; cytotoxicity; gingival fibroblasts; cell viability; apoptosis</p>