Cognitive Performance and Social Interaction on Nicotine-preferred Rat Lines
Project no. 23456 · Status: Kapanmış (Yayın bekleniyor) · 2021
Summary
<p>AIM: The fact that tobacco use is one of the most important health problems worldwide creates a need to address nicotine addiction with all its dimensions. In this context, A nicotine-preferring (NP) rat line was developed to investigate the effects and new therapies for nicotine addiction. Aim of the present study was to investigate the changes in cognitive performance and social interaction of rats with nicotine-preference.</p><p>METHODS: Forty-eight Sprague-Dawley rats were assigned to four groups: Nicotine-preferring male (NPM),nicotine-preferring female (NPF), control male (CM) and control female (CF). New object recognition (NOR), object location recall (OLT) and Morris Water Maze (MWM) tests were used to evaluate the cognitive performance; three-chamber social interaction test (TCSI) was used to evaluate the social interactions. Data were analyzed with ANOVA tests using IBM SPSS 20.00 software.</p><p>RESULTS: NP rats had increased interest in objects (F(3.38)=5.128 P=0.030) in the NOR familiarization session.All groups were more interested in the new object in the test session (F(1.78)= 20.524 P=0.000). Line differences were found in total time spent with the objects in the OLT familiarization session (P=0.000), time spent with the displaced object in test session (P=0.000) and in discrimination index (P=0.028). During MWM spatial learning acquisition period, time to reach the platform decreased by days. The effect of sex (p=0.025) and line (p=0.009) was statistically significant, but there was no interaction between them. In Duncan’s test showed a significant difference between CF group and NPM and NPF groups. In probe trial, there was also a significant effect of line on path taken in the old quadrant (p=0.001).NP rats spent longer time in the old quadrant.No significant differences in line were found in social interaction and social novelty tests.</p><p>CONCLUSIONS: Nicotine-preferring rats have an advantage in detecting new objects and locations and they have better spatial memory.However, they displayed no significant change in social interaction and have no sex difference. We hope that these results will improve our understanding of addiction (project number: DESTEK BAP T TYL–2021–23456 EUHADYEK 2021-074)</p><p>Keywords: memory; nicotine; learning; social interaction; sexual differences</p>