DEVELOPMENT OF ELECTROCHEMICAL BIOSENSOR SYSTEM FOR MEASURING HYPOXIA INDUCICLE FACTOR-1 ALPHA LEVEL W IN BLOOD
Project no. 182 · Status: Kapanmış · 2018
Summary
Hypoxia is a toxic condition caused by a decrease in oxygen content in the body tissues. In this case, many metabolic activities in the body are changing and the concentrations of markers that can show hypoxia status can be increased. In studies, hypoxia-inducible factor-1 (HIF-1?) was found to be a key factor in the development of the adaptation response to hypoxia. HIF-1 is the main regulator of oxygen homeostasis; responsible for angiogenesis, erythropoiesis, metabolism, proliferation, migration and adaptive cellular response to hypoxia to regulate cell life. In this study; tissue hypoxia-inducible factor-1 levels may show tissue oxygenation as a parameter indicating tissue oxygenation in patients who had diabetic foot injury and diabetic foot wound which is a common complication of Diabetes Mellitus and participated in Council of Diabetic Foot. A new method was developed for the measurement of HIF-1? by the electrochemical impedance spectroscopy (EIS) based biosensor system. In this method, HIF-1alpha antibodies have been used as a biota receptor. For this immobilization, the electrode was first modified with albumin, followed by PAMAM. According to the chronoimpedance data, the total analysis time for EIS was chosen as 15 minutes. The new biosensor was compared with the ELISA method requiring more than 5 hours. Calibration curve of the electrode electrode transfer resistance ohm and HIF1 biosensor between 50 pg/mL to 1000 pg / mL calibration curve was obtained. R2 value was found to be 0.9964. LOD and LOQ of the biosensor were calculated as 14.45 pg/mL, 43.8 pg/mL, respectively.