Characterization of Mesenchymal Stem Cells Isolated from Wharton Gelly
Project no. 21207 · Status: Kapanmış · 2019
Summary
Stem cells are precursor cells that can be found in all tissues in the human body. It has been shown that these undifferentiated cells have the ability to unlimitedly divide, regenerate themselves and differentiate into different cell types. In line with the latest developments in science and technology, the use of stem cells in cancer, nervous system diseases and damages, metabolic diseases, rheumatic diseases, heart diseases, bone diseases and many other areas has come to the fore. Research on stem cells, which offer great promise in terms of regenerative medicine, continues rapidly at both laboratory and clinical levels. Although embryonic stem cells are very good resources in terms of their high differentiation potential, ethical problems related to the use of embryonic tissues and their neoplastic potential (teratoma) have restricted their use; and alternative stem cell sources, which are called adult stem cells and obtained from somatic tissues, especially mesenchymal stem cells (MSCs) have gained importance. Mesenchymal stem cells isolated from Wharton’s jelly (WJ-MSCs) are a recently discovered type of mesenchymal stem cell with multiple lineage potential. WJ-MSCs are advantageous regenerative cell candidates for tissue engineering since they can be isolated in high numbers with low damage, and they Show lower immunogenicity than other adult mesenchymal stem cells. With this study, which was carried out with the aim of establishing a basis for potential use of WJ-MSCs in the field of regenerative medicine, the protocol for obtaining mesenchymal stem cells isolated from rabbit Wharton’a jelly was optimized and the cells obtained were characterized. According to experimental results, isolated cells express CD29, CD90, CD54 and MHCI positive markers, while they do not express CD11b, CD34, CD45 and MHCII negative markers, proving that WJ-MSCs were succesfully isolated.