EVALUATİON OF PLANT GROWTH PROMOTING BACTERIA (PGPB) IN POTATO CULTIVATION IN TERMS OF SUSTAINABLE AGRICULTURE
Project no. 27372 · Status: Kapanmış (Yayın bekleniyor) · 2023
Summary
<p>Plant growth-promoting bacteria (PGPB) are gaining increasing significance in sustainable agriculture as an eco-friendly approach to enhance agricultural productivity and reduce chemical inputs. This thesis aims to investigate the impact of diseases on soil microbiota and the resulting effects on plant growth by comparing the diversity and abundance of PGPB species in fields where healthy and diseased potatoes were grown. In this context, on October 10, 2023, plant and soil samples were collected from five different points in two distinct potato fields —one exhibiting healthy plant growth and the other showing diseased plants—in the Ödemiş district of İzmir, Turkey. These fields were selected based on information obtained from local farmers and fertilizer suppliers. Physicochemical analyses were performed on soil samples, including the measurement of parameters such as temperature, oxygen levels, pH, and electrical conductivity, as well as the quantification of key elements, namely nitrogen (N), phosphorus (P), iron (Fe), manganese (Mn), and arsenic (As). A total of 65 endophytic bacterial isolates were obtained from the plant samples, and their phenotypic and microscopic characteristics were carried out. The isolates were further evaluated for plant growth-promoting traits such as phosphate solubilization, ACC deaminase activity, IAA production, ammonia production, and siderophore production. Based on these evaluations, 10 isolates exhibiting the best performance were selected for 16S rRNA analysis to identify their species. The analysis revealed that these isolates belonged to genera such as x Acinetobacter, Chryseobacterium, Pantoea, Pseudomonas, Enterobacter, Klebsiella, and Bacillus. The study concluded that bacteria isolated from healthy potato fields exhibited higher plant growth-promoting biochemical activities compared to those isolated from diseased fields. The findings of this thesis contribute to understanding the relationship between PGPB mechanisms and soil health and are expected to guide future research in this area.</p>