Research on the biosynthesis of metallic nanoparticles using Cephalaria plant extracts and their biopharmaceutical applications.
Project no. 20292 · Status: Kapanmış · 2019
Summary
The eco-friendly biological methods of nanomaterials production to be applied against human cancer cells, became a target of current scientific studies. Being a part of that, and by framing the unreported mechanism occuring during the synthesis of green nanoparticle using plant extracts, Cephalaria tchihatchewii plant was aimed to be extracted in different organic solvents such as water, methanol, butanol, and a mixture of saponins, followed by some isolation and purification processes of various compounds using several chromatographic applications (TLC, VLC, MPLC, CC). Two novel glycosidic compounds Tchihatchewoside A-B, along with 13 known compounds were isolated, and characterized by 1D,2D NMR Spectroscopy. Using the phytobiological (co-precipitation) method, the cited four extracts along with, one saponin, one iridoid, one methyl glycoside, and one flavonoid were reacted with silver and zinc metallic solutions and produce various nanoparticles mostly found in the nanoscale range after been characterized with UV-Vis spectrophotometer, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and the Fourier Transmission Infra-Red (FTIR). The cytotoxic activities of produced nanoparticles on A549, MDA MB, PC3, and CCD 34Lu cell lines, were evaluated by the MTT method using doxorubicin as a positive control and showed moderate cytotoxicity of five nanoparticles such as water-zinc, water-silver, methanol-zinc, methanol-silver, and saponins-zinc. Whereas both novel triterpenoid saponins didn’t show any considerable cytotoxic activity against CD 34Lu, A549, CRL5807, CRL 5826, HTB-177 and HeLa cells. IL1ß cytokine release was stimulated by both novel compounds. In terms of pure compounds-nanoparticles; only silver nitrate and the respective silver mediated nanoparticles display a significant cytotoxicity even higher than the positive control for some of them. The in-vitro antioxidant and anti-inflammatory activities of produced nanoparticles were evaluated using DPPH and inhibition of protein denaturation assays respectively.