Effects of Feed Restriction on Performance, Some Blood, Organ and Bone Parameters of Rats
Project no. 1128 · Status: Kapanmış · 2018
Summary
In this study, 3 groups were formed to apply ad libitum (control), once a week (1G) and two days a week (2G) to apply feed restriction. Each group contains 5 female and 5 male rats, which are individually housed in metabolism cages. The trial was continued for 3 months. At the end of the trial, parameters of live weight, live weight gain, feed consumption, feed utilization and mortality of rats were evaluated. Liver enzymes in blood samples taken from rats; Aspartate amino transferase (AST), alanine aminotransferase (ALT), blood serum cholesterol, triglyceride, HDL (high density lipoprotein), LDL (low density lipoprotein) values, cortisol and seratonin values, and crude ash, calcium and phosphorus values in tibia samples. In addition, organ weights and proportional values of rats (heart, liver, spleen, kidney, stomach, pancreas and intestines) and liver fat content and amount of malondialdehyde (MDA) were determined. While the live weights were determined that at the beginning of the trial in the control, 1G and 2G groups, respectively, 264.20, 253.80 and 265.20 g, at the end of the trial, the live weights were 364.80, 331.60 and 324.90 g. While there was no statistical difference between the groups (P>0.05), it was determined that the sex significantly affected the live weights (P<0.05). When the feed consumption was examined, in the control 1G and 2G groups, respectively were determined as 2055.04, 2015.05, 1734.69 g and it was not found statistically significant (P>0.05). It has been determined that the sex has a significant effect on feed consumption (P<0.05). At the end of the experiment there was no statistical difference between the groups in terms of internal organ weights (heart, liver, spleen, kidney, pancreas, stomach, intestines and their relative values) (P<0.05), but the effect of the sex on the liver, kidney and intestinal weight and their relative values were statistical was found to be significant (P<0.05). When the blood parameters were examined (seratonin, cortisol, AST, ALT, cholesterol, HDL, LDL and triglyceride), no statistical difference was found between the groups (P>0.05). The effect of the sex was determined to be significant on the LDL and trigiliseride values (P<0.05).