Preparation of Polysaccharide-Reinforced Collagen and Keratin-Based Biomaterials Containing Chaste Extract and Determination of the Wound Healing Potential
Project no. 25234 · Status: Kapanmış (Yayın bekleniyor) · 2023
Summary
<p>The project was aimed to develop collagen-based biomaterials reinforced with polysaccharides and keratin, crosslinked with <em>Vitex agnus-castus</em> extract, and to assess their efficacy in wound healing. In the form of film and sponges, one hundred and one biomaterials were synthesized, using various polysaccharides such as gum arabic, carboxymethyl cellulose, carrageenan, microcrystalline cellulose, soy protein and keratin, crosslinked with either <em>Vitex agnus castus </em>extract or glutaraldehyde.</p><p><br></p><p>The morphological, structural, thermal, mechanical, and biological characteristics of the collagen-based biomaterials were assessed using FT-IR, DSC, TGA, water absorption capacity, antibacterial activity, <em>in vitro</em> cell culture studies, and an <em>in vivo</em> wound healing model subsequent to formulation application. The findings indicated that using Vitex extract as a crosslinking agent, especially in conjunction with carrageenan and gum arabic, improved the structural integrity and biological efficacy of the materials. Significant disparities were noted between the film and sponge forms for water absorption capacity and antibacterial efficacy; sponge-type biomaterials demonstrated more water uptake but, consequently, reduced water retention duration compared to films, in addition to a wider antibacterial spectrum.</p><p><br></p><p>Furthermore, assays for cell survival and proliferation were performed to ascertain the appropriate dose of Vitex extract. Considering these results, chosen film samples underwent initial assessments in wound healing models. The findings suggest that Vitex extract functions as a biocompatible and effective crosslinker, whereas collagen-based biomaterials have significant promise for wound healing applications. The work significantly contributes to the literature by demonstrating the possible use of iridoid-containing plants in biomaterial production.</p>