DEVELOPMENT OF THE DERMATOLOGICAL FORM BY USING THE MIXTURE OF OLIVE OIL AND Momordica charantia L. WHICH IS TRADITIONALLY USED AS WOUND HEALING AGENT AND INVESTIGATION OF IN VITRO WOUND-HEALING EFFECT
Project no. 1121 · Status: Kapanmış · 2018
Summary
This study was designed for preparation of wound-healing cream form from the mixture of olive oil and Momordica charantia, used as wound healing agent. We also aimed to employ the standardization and quality control studies of prepared cream form and to investigate its wound healing effect using in vitro cell culture method. Within the scope of this study, first a mixture of olive oil and Momordica charantia was prepared. Total flavonoid contents of prepared cream form were determined by using spectrophotometry and high pressure liquid chromatography. Quality control studies of this cream were studied in vitro to determine the total amount of flavonoid, pH, viscosity and stability. Biological activities of prepared cream form were studied to determine the cell migration and wound healing effect on BJ and HaCaT cells by in vitro cell culture. The laboratory scale cream form, prepared using the mixture of olive oil and Momordica charantia together with pharmaceutical quality excipients, was standardized on the total flavonoid content. The product was found to be stable at 40°C with 60% humidity. In in vitro wound healing models of BJ and HaCaT cells with increasing doses of cream form, cell migration was observed to increase, compared to control group. Keywords: Momordica charantia, wound-healing effect, flavonoid, cream