Effect of Blood Flow Restricted Exercise on Acute Systemic Irisin, Myostatin and Decorin Levels
Project no. 24172 · Status: Kapanmış (Yayın bekleniyor) · 2022
Summary
<p>Blood flow restricted resistance exercise is a relatively new exercise method used to increase skeletal muscle hypertrophy. It has been reported that low-load resistance exercises with restricted blood flow (BFR-RE) produce skeletal muscle hypertrophy similar to traditional high-load resistance exercises (HL-RE). Myokines are cytokines that are synthesized in skeletal muscle and released into the blood in response to contraction. Irisin, myostatin and decorin are myokines that have been shown to play a role in skeletal muscle hypertrophy. Therefore, in our study, we investigated the effects of BFR-RE and traditional HL-RE on acute systemic irisin, myostatin and decorin levels. Our hypothesis is that the changes in irisin, myostatin and decorin levels after both exercises will be similar. Healthy, physically active and young (23±2 years) male participants were included in the study. Participants were randomized to a single session of BFR-RE and HL-RE and completed both exercise sessions approximately one week apart. Bilateral knee extension exercise was performed in both sessions. BFR-RE was performed in 30% of 1-TM, as a set of 30 repetitions followed by three sets of 15 repetitions, with a one-minute rest period between sets. Blood flow restriction was performed through a cuff placed on the proximal thigh bilaterally, and the pressure corresponding to 50% of the AOP was determined as the cuff pressure. HL-RE was performed in 80% of 1-TM, as four sets of 7 repetitions, with two minutes rest between sets. These parameters were selected in accordance with the skeletal muscle hypertrophy guidelines and care was taken to ensure that the total exercise volumes were as equal as possible. Plasma irisin, myostatin and decorin concentrations were measured by ELISA method in blood samples taken before and immediately after exercise. While there was a statistically significant increase in plasma irisin level (p=0.009) and decorin level (p=0.036) after BFR-RE, we found a significant decrease in plasma myostatin level (p=0.048). There was no significant difference in plasma irisin and myostatin levels (p=0.160 and p=0.084, respectively) after HL-RE compared to pre-exercise, but the difference in plasma decorin levels was significant (p=0.002). Considering the effect size, the change in plasma decorin levels was similar in both exercise types. BFR-RE had a greater effect on the change in irisin and myostatin levels. The change in decorin level was similar in both exercises. We think that these changes in irisin, myostatin and decorin myokines may contribute to skeletal muscle hypertrophy.</p>