KIZILÇAM ÖZÜTÜ İLE YARA ÖRTÜSÜ AMAÇLI TEKSTİL YÜZEYLERİNİN OLUŞTURULMASI
Project no. 697 · Status: Kapanmış · 2015
Summary
Wound dress which has largest share of medical textiles, is one of the significant applications. Studies carried out for wound healing purposes date back to ancient years. Although it has been used for many years, Pine bark has recently a wide area of application in nutrition health and medicine. The aim of this project is to design wound dressings using the extracts of Pinus brutia known as Turkish Pine, and to determine the wound healing performances of the obtained products in vivo experiments. Pinus brutia extracts were obtained using modern extraction techniques such as microwave and ultrasonic extraction apart from the soxhlet extraction method. Optimum extraction conditions obtained using with Box-Bhenken design were elicited as 70ºC, 10 min., 900 W which yielded 987.32 mg gallic acid equivalent (GAE), 23.7 mg quercetin/g extract and 86.2% free radical scavenging capacity for microwave-assisted water/ethylacetate extraction; while 70 ºC, 20 min. and %50 power which yielded 811.84 mg gallic acid equivalent (GAE), 30.1 mg quercetin/g extract and 90.8% free radical scavenging capacity for ultrasonically-assisted water-ethylacetate extraction. After the extraction, the sodium alginate solution was prepared using Pinus Brutia extract and the gel was formed on the fabric by treatment CaCl2 solution to obtain wound dress. Wound healing performances of the wound dressings obtained using with extracts were tested on rats. At the end of the first week, 75.7 percent wound area of rats which treated with wound dress containing Pinus brutia, was healed, while in control group rats 48.6 percent wound area was healed.