Identification of ppp2ca Gene Variations and Association Analyses with Growth Traits in European Seabass (Dicentrarchus labrax)

Project no. 33386 · Status: Kapanmış · 2025

Summary

<p> The&nbsp;<em>ppp2cab</em>&nbsp;gene&nbsp;encodes&nbsp;the&nbsp;catalytic&nbsp;alpha&nbsp;subunit&nbsp;of&nbsp;protein&nbsp;phosphatase&nbsp;2A&nbsp;(PP2A),&nbsp;which&nbsp;plays&nbsp;an&nbsp;important&nbsp;role&nbsp;in&nbsp;the&nbsp;regulation&nbsp;of&nbsp;cellular&nbsp;growth,&nbsp;proliferation,&nbsp;and&nbsp;various&nbsp;cellular&nbsp;processes.&nbsp;However,&nbsp;the&nbsp;relationship&nbsp;between&nbsp;genetic&nbsp;variations&nbsp;in&nbsp;this&nbsp;gene&nbsp;and&nbsp;growth&nbsp;traits&nbsp;in&nbsp;European&nbsp;seabass&nbsp;(<em>Dicentrarchus&nbsp;labrax</em>)&nbsp;has&nbsp;not&nbsp;been&nbsp;sufficiently&nbsp;investigated.&nbsp;This&nbsp;study&nbsp;aimed&nbsp;to&nbsp;identify&nbsp;single&nbsp;nucleotide&nbsp;polymorphisms&nbsp;(SNPs)&nbsp;in&nbsp;a&nbsp;partial&nbsp;region&nbsp;of&nbsp;the&nbsp;<em>ppp2cab</em>&nbsp;gene&nbsp;in&nbsp;European&nbsp;seabass,&nbsp;evaluate&nbsp;the&nbsp;associations&nbsp;of&nbsp;these&nbsp;variants&nbsp;with&nbsp;growth&nbsp;traits,&nbsp;and&nbsp;investigate&nbsp;the&nbsp;potential&nbsp;effects&nbsp;of&nbsp;changes&nbsp;in&nbsp;the&nbsp;coding&nbsp;region&nbsp;on&nbsp;protein&nbsp;structure&nbsp;through&nbsp;in&nbsp;silico&nbsp;analyses.&nbsp;A&nbsp;total&nbsp;of&nbsp;seven&nbsp;SNPs,&nbsp;three&nbsp;exonic&nbsp;and&nbsp;four&nbsp;intronic,&nbsp;were&nbsp;identified&nbsp;in&nbsp;the&nbsp;target&nbsp;gene&nbsp;region.&nbsp;Two&nbsp;of&nbsp;the&nbsp;exonic&nbsp;SNPs&nbsp;were&nbsp;found&nbsp;to&nbsp;be&nbsp;nonsynonymous&nbsp;variants&nbsp;resulting&nbsp;in&nbsp;the&nbsp;Ala102Pro&nbsp;and&nbsp;Asp151Asn&nbsp;amino&nbsp;acid&nbsp;substitutions,&nbsp;while&nbsp;the&nbsp;third&nbsp;was&nbsp;identified&nbsp;as&nbsp;the&nbsp;synonymous&nbsp;g.7857G&gt;A&nbsp;variant,&nbsp;which&nbsp;does&nbsp;not&nbsp;alter&nbsp;the&nbsp;amino&nbsp;acid&nbsp;sequence.&nbsp;The&nbsp;associations&nbsp;between&nbsp;the&nbsp;polymorphisms&nbsp;and&nbsp;body&nbsp;weight,&nbsp;fillet&nbsp;weight,&nbsp;standard&nbsp;length,&nbsp;and&nbsp;various&nbsp;morphometric&nbsp;traits&nbsp;were&nbsp;statistically&nbsp;evaluated.&nbsp;The&nbsp;results&nbsp;revealed&nbsp;that&nbsp;only&nbsp;the&nbsp;g.7857G&gt;A&nbsp;SNP&nbsp;showed&nbsp;significant&nbsp;associations&nbsp;with&nbsp;the&nbsp;examined&nbsp;growth&nbsp;and&nbsp;morphometric&nbsp;traits&nbsp;(p&nbsp;&lt;&nbsp;0.05).&nbsp;Structural&nbsp;analyses&nbsp;indicated&nbsp;that&nbsp;the&nbsp;Ala102Pro&nbsp;and&nbsp;Asp151Asn&nbsp;substitutions&nbsp;are&nbsp;located&nbsp;outside&nbsp;the&nbsp;predicted&nbsp;catalytic&nbsp;center&nbsp;of&nbsp;the&nbsp;protein&nbsp;and&nbsp;are&nbsp;not&nbsp;expected&nbsp;to&nbsp;substantially&nbsp;alter&nbsp;its&nbsp;overall&nbsp;structure.&nbsp;In&nbsp;conclusion,&nbsp;this&nbsp;study&nbsp;revealed&nbsp;genetic&nbsp;diversity&nbsp;in&nbsp;the&nbsp;<em>ppp2cab</em>&nbsp;gene&nbsp;in&nbsp;European&nbsp;seabass&nbsp;and&nbsp;evaluated&nbsp;the&nbsp;potential&nbsp;associations&nbsp;of&nbsp;the&nbsp;identified&nbsp;polymorphisms&nbsp;with&nbsp;growth&nbsp;traits.&nbsp;The&nbsp;findings&nbsp;are&nbsp;expected&nbsp;to&nbsp;provide&nbsp;a&nbsp;basis&nbsp;for&nbsp;validation&nbsp;and&nbsp;functional&nbsp;studies&nbsp;in&nbsp;different&nbsp;populations&nbsp;and&nbsp;contribute&nbsp;to&nbsp;understanding&nbsp;the&nbsp;genetic&nbsp;basis&nbsp;of&nbsp;growth&nbsp;traits&nbsp;in&nbsp;this&nbsp;species.&nbsp;Furthermore,&nbsp;the&nbsp;identified&nbsp;variants&nbsp;provide&nbsp;valuable&nbsp;preliminary&nbsp;data&nbsp;for&nbsp;the&nbsp;characterization&nbsp;of&nbsp;genetic&nbsp;resources,&nbsp;the&nbsp;molecular&nbsp;elucidation&nbsp;of&nbsp;growth&nbsp;performance,&nbsp;and&nbsp;the&nbsp;development&nbsp;of&nbsp;future&nbsp;genetics-based&nbsp;breeding&nbsp;applications&nbsp;in&nbsp;this&nbsp;economically&nbsp;important&nbsp;species.&nbsp;However,&nbsp;further&nbsp;studies&nbsp;involving&nbsp;larger&nbsp;sample&nbsp;sizes&nbsp;are&nbsp;required&nbsp;to&nbsp;establish&nbsp;these&nbsp;associations&nbsp;reliably.</p>

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