Increasing the Efficiency of Stem Cell Harvesting from Adipose Tissue via Ultrasonic Adipolysis

Project no. 26328 · Status: Kapanmış (Yayın bekleniyor) · 2022

Summary

<p>In&nbsp;recent&nbsp;years,&nbsp;mesenchymal&nbsp;stem&nbsp;cells&nbsp;have&nbsp;emerged&nbsp;as&nbsp;a&nbsp;promising&nbsp;therapeutic&nbsp;agent&nbsp;due&nbsp;to&nbsp;their&nbsp;differentiation&nbsp;capabilities,&nbsp;utility&nbsp;in&nbsp;tissue&nbsp;engineering,&nbsp;and&nbsp;paracrine&nbsp;support&nbsp;for&nbsp;tissue&nbsp;regeneration.&nbsp;Adipose-derived&nbsp;stem&nbsp;cells&nbsp;(ADSCs)&nbsp;have&nbsp;become&nbsp;a&nbsp;significant&nbsp;source&nbsp;for&nbsp;therapies&nbsp;utilizing&nbsp;mesenchymal&nbsp;stem&nbsp;cells,&nbsp;owing&nbsp;to&nbsp;their&nbsp;relative&nbsp;abundance&nbsp;and&nbsp;ease&nbsp;of&nbsp;isolation&nbsp;from&nbsp;adipose&nbsp;tissue.</p><p>Various&nbsp;methods&nbsp;have&nbsp;been&nbsp;investigated&nbsp;for&nbsp;isolating&nbsp;adipose-derived&nbsp;stem&nbsp;cells,&nbsp;and&nbsp;liposuction&nbsp;has&nbsp;been&nbsp;reported&nbsp;as&nbsp;a&nbsp;safe&nbsp;procedure&nbsp;for&nbsp;this&nbsp;purpose.&nbsp;Furthermore,&nbsp;technological&nbsp;advancements&nbsp;have&nbsp;led&nbsp;to&nbsp;the&nbsp;development&nbsp;of&nbsp;various&nbsp;liposuction&nbsp;techniques&nbsp;that&nbsp;minimize&nbsp;donor-site&nbsp;morbidity&nbsp;without&nbsp;compromising&nbsp;the&nbsp;yield&nbsp;or&nbsp;efficacy&nbsp;of&nbsp;the&nbsp;harvested&nbsp;stem&nbsp;cells.&nbsp;One&nbsp;such&nbsp;technique&nbsp;is&nbsp;ultrasound-assisted&nbsp;liposuction.&nbsp;Studies&nbsp;have&nbsp;demonstrated&nbsp;that&nbsp;the&nbsp;yield,&nbsp;viability,&nbsp;and&nbsp;differentiation&nbsp;potential&nbsp;of&nbsp;ADSCs&nbsp;obtained&nbsp;via&nbsp;ultrasonic&nbsp;liposuction&nbsp;are&nbsp;comparable&nbsp;to&nbsp;those&nbsp;obtained&nbsp;through&nbsp;conventional&nbsp;methods,&nbsp;confirming&nbsp;the&nbsp;safety&nbsp;of&nbsp;this&nbsp;technique&nbsp;for&nbsp;stem&nbsp;cell&nbsp;isolation.</p><p>&nbsp;Although&nbsp;various&nbsp;liposuction&nbsp;techniques&nbsp;have&nbsp;been&nbsp;described,&nbsp;it&nbsp;is&nbsp;impossible&nbsp;to&nbsp;harvest&nbsp;the&nbsp;entirety&nbsp;of&nbsp;the&nbsp;adipose&nbsp;tissue&nbsp;from&nbsp;the&nbsp;target&nbsp;area&nbsp;regardless&nbsp;of&nbsp;the&nbsp;technique&nbsp;employed;&nbsp;significant&nbsp;amounts&nbsp;of&nbsp;adipose&nbsp;tissue&nbsp;remain&nbsp;surrounding&nbsp;the&nbsp;stromal&nbsp;connective&nbsp;tissue&nbsp;in&nbsp;the&nbsp;abdominoplasty&nbsp;specimen&nbsp;following&nbsp;liposuction.&nbsp;Typically,&nbsp;the&nbsp;abdominoplasty&nbsp;specimen&nbsp;is&nbsp;discarded&nbsp;after&nbsp;these&nbsp;procedures.&nbsp;We&nbsp;hypothesized&nbsp;that&nbsp;this&nbsp;residual&nbsp;adipose&nbsp;tissue—which&nbsp;is&nbsp;not&nbsp;harvested&nbsp;during&nbsp;conventional&nbsp;or&nbsp;ultrasonic&nbsp;liposuction—contains&nbsp;a&nbsp;higher&nbsp;concentration&nbsp;of&nbsp;ADSCs&nbsp;per&nbsp;unit&nbsp;compared&nbsp;to&nbsp;the&nbsp;lipoaspirate.&nbsp;In&nbsp;our&nbsp;study,&nbsp;involving&nbsp;patients&nbsp;undergoing&nbsp;abdominoplasty&nbsp;combined&nbsp;with&nbsp;ultrasonic&nbsp;liposuction...&nbsp;We&nbsp;aim&nbsp;to&nbsp;demonstrate&nbsp;that&nbsp;the&nbsp;number&nbsp;and&nbsp;yield&nbsp;of&nbsp;adipose&nbsp;tissue-derived&nbsp;stem&nbsp;cells&nbsp;per&nbsp;unit&nbsp;are&nbsp;higher&nbsp;in&nbsp;the&nbsp;residual&nbsp;perivascular&nbsp;immobile&nbsp;adipose&nbsp;tissue—which&nbsp;remains&nbsp;after&nbsp;liposuction&nbsp;and&nbsp;cannot&nbsp;be&nbsp;removed&nbsp;by&nbsp;the&nbsp;procedure—compared&nbsp;to&nbsp;the&nbsp;tissue&nbsp;obtained&nbsp;via&nbsp;liposuction&nbsp;methods.</p>

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