CHARACTERIZATION, TOXICITY AND EFFICACY POTENTIAL OF ? - POLYGLUTAMIC ACID (? -PGA) NANOPARTICLES
Project no. 611 · Status: Kapanmış · 2015
Summary
Nanomaterials can be physically and chemically modified and have been used commonly in recent years with their widespread use in many areas as medicine, biomedical and industry. p(HEMA) was obtained with surfactant free emulsion polymerization method and it was synthesized with L-glutamic acid by grafting technique. Characterization analysis of polymeric nanoparticles were investigated by SEM (Scanning Electron Microscopy) imaging to determine their size and morphological characteristics, ZETA size-potential analysis to determine their behavior in polar liquids, AFM (Atomic Force Microscopy) analysis to display surface topography and their interactions with surface chemistry by FTIR Absorption Spectroscopy. According to the results of characterization analysis, L-glutamic acid-g-p (HEMA) has 194.6 nm size and -18 Mv negative surface value. The toxicity of polymeric nanoparticle was determined with in vitro and in vivo assays. L-glutamic acid-g-p (HEMA) has no cytotoxic effect on 16HBE (Human bronchial epithelial cell line) and THP-1 (human monocytic cell line) cell lines with WST-1 and LDH tests and in order to determine the mutagenic potential on Salmonella typhimurium strains which was not showed mutagenic effect with Salmonella/AMES test. In order to determine genotoxic potential, it has been identified that there is no genotoxic effect with COMET test on 16HBE and THP-1 cell lines and in vivo micronucleus test on bone marrow. Cell migration test was performed on 16HBE cell line and the percent closure rate was determined as 21%. The irritation potential was obtained with applied concentration by HET-CAM test and antimicrobial activity were determined with MIC method. The LD50 value was determined as ? 2000 mg / kg by the in vivo single dose acute toxicity test, also biochemical data and histopathological investigation were obtained with the animal and organ weight data as a nontoxic result of the 28 days repeated dose toxicity (subacute) study. The biodistribution of the polymeric nanoparticle in the body was demonstrated by IVIS spectrum which is in vivo imaging system. As a result, L-glutamic acid-g-p (HEMA), a polymeric nanoparticle which was synthesized and characterized by us, has been found to be biocompatible and has the potential to be used as a drug delivery system.