Production Of Fibrin Sealant-Based Biological Contact Lenses Functionalized For Drug Release

Project no. 32672 · Status: Kapanmış (Yayın bekleniyor) · 2025

Summary

<p>Frequent&nbsp;administration&nbsp;of&nbsp;antibiotic&nbsp;eye&nbsp;drops&nbsp;for&nbsp;the&nbsp;treatment&nbsp;of&nbsp;corneal&nbsp;infections&nbsp;may&nbsp;reduce&nbsp;patient&nbsp;adherence&nbsp;and&nbsp;increase&nbsp;treatment&nbsp;burden.&nbsp;In&nbsp;this&nbsp;study,&nbsp;a&nbsp;personalized&nbsp;fibrin&nbsp;sealant-based&nbsp;biological&nbsp;contact&nbsp;lens&nbsp;was&nbsp;developed&nbsp;for&nbsp;controlled&nbsp;vancomycin&nbsp;delivery,&nbsp;and&nbsp;its&nbsp;in&nbsp;vitro&nbsp;feasibility&nbsp;was&nbsp;evaluated.</p><p>Ocular&nbsp;surface&nbsp;molds&nbsp;obtained&nbsp;from&nbsp;postmortem&nbsp;small-ruminant&nbsp;eyes&nbsp;collected&nbsp;from&nbsp;a&nbsp;slaughterhouse&nbsp;were&nbsp;digitized&nbsp;using&nbsp;optical&nbsp;three-dimensional&nbsp;(3D)&nbsp;scanning,&nbsp;processed&nbsp;with&nbsp;computer-aided&nbsp;design&nbsp;software,&nbsp;and&nbsp;used&nbsp;to&nbsp;fabricate&nbsp;personalized&nbsp;molds&nbsp;by&nbsp;stereolithography&nbsp;(STL).&nbsp;Vancomycin-loaded&nbsp;fibrin&nbsp;sealant-based&nbsp;biological&nbsp;contact&nbsp;lenses&nbsp;were&nbsp;subsequently&nbsp;prepared&nbsp;using&nbsp;these&nbsp;molds.&nbsp;The&nbsp;structural&nbsp;integrity&nbsp;of&nbsp;the&nbsp;system&nbsp;and&nbsp;its&nbsp;vancomycin&nbsp;release&nbsp;profile&nbsp;were&nbsp;evaluated&nbsp;over&nbsp;72&nbsp;h&nbsp;in&nbsp;distilled&nbsp;water&nbsp;and&nbsp;phosphate-buffered&nbsp;saline&nbsp;(PBS).</p><p>The&nbsp;developed&nbsp;manufacturing&nbsp;approach&nbsp;enabled&nbsp;the&nbsp;successful&nbsp;fabrication&nbsp;of&nbsp;biological&nbsp;contact&nbsp;lenses&nbsp;conforming&nbsp;to&nbsp;ocular&nbsp;surface&nbsp;anatomy,&nbsp;while&nbsp;the&nbsp;lenses&nbsp;maintained&nbsp;their&nbsp;overall&nbsp;structural&nbsp;integrity&nbsp;throughout&nbsp;the&nbsp;evaluation&nbsp;period.&nbsp;Vancomycin&nbsp;exhibited&nbsp;a&nbsp;biphasic&nbsp;release&nbsp;profile&nbsp;in&nbsp;both&nbsp;media.&nbsp;Cumulative&nbsp;release&nbsp;during&nbsp;the&nbsp;first&nbsp;24&nbsp;h&nbsp;reached&nbsp;52.40%&nbsp;in&nbsp;distilled&nbsp;water&nbsp;and&nbsp;50%&nbsp;in&nbsp;PBS,&nbsp;increasing&nbsp;to&nbsp;59.22%&nbsp;and&nbsp;77%,&nbsp;respectively,&nbsp;at&nbsp;72&nbsp;h.&nbsp;These&nbsp;findings&nbsp;indicate&nbsp;an&nbsp;initial&nbsp;rapid&nbsp;release&nbsp;phase&nbsp;followed&nbsp;by&nbsp;a&nbsp;slower,&nbsp;more&nbsp;controlled&nbsp;release&nbsp;phase.</p><p>In&nbsp;conclusion,&nbsp;personalized&nbsp;fibrin&nbsp;sealant-based&nbsp;biological&nbsp;contact&nbsp;lenses&nbsp;can&nbsp;be&nbsp;fabricated&nbsp;using&nbsp;three-dimensional&nbsp;modeling&nbsp;and&nbsp;printing&nbsp;technologies&nbsp;and&nbsp;can&nbsp;provide&nbsp;vancomycin&nbsp;release&nbsp;for&nbsp;at&nbsp;least&nbsp;72&nbsp;h.&nbsp;This&nbsp;approach&nbsp;provides&nbsp;a&nbsp;promising&nbsp;basis&nbsp;for&nbsp;further&nbsp;formulation&nbsp;optimization&nbsp;and&nbsp;in&nbsp;vivo&nbsp;evaluation&nbsp;of&nbsp;controlled&nbsp;ocular&nbsp;antibiotic&nbsp;delivery&nbsp;systems.</p><p><strong>Keywords:</strong>&nbsp;Infectious&nbsp;keratitis;&nbsp;fibrin&nbsp;sealant;&nbsp;controlled&nbsp;release;&nbsp;biological&nbsp;contact&nbsp;lens.</p>

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