Design of Bioluminescent Based Microbial Biosensor as a Pesticide Detector in Sustainable Agriculture

Project no. 25241 · Status: Kapanmış (Yayın bekleniyor) · 2022

Summary

<p>Pesticides&nbsp;are&nbsp;widely&nbsp;used&nbsp;in&nbsp;agricultural&nbsp;production&nbsp;to&nbsp;increase&nbsp;yield;&nbsp;however,&nbsp;they&nbsp;may&nbsp;accumulate&nbsp;in&nbsp;the&nbsp;environment&nbsp;and&nbsp;cause&nbsp;adverse&nbsp;effects&nbsp;on&nbsp;ecosystem&nbsp;and&nbsp;human&nbsp;health.&nbsp;Therefore,&nbsp;the&nbsp;rapid,&nbsp;sensitive&nbsp;and&nbsp;reliable&nbsp;detection&nbsp;of&nbsp;pesticide&nbsp;residues&nbsp;in&nbsp;environmental&nbsp;matrices&nbsp;is&nbsp;of&nbsp;great&nbsp;importance.&nbsp;Although&nbsp;conventional&nbsp;analytical&nbsp;methods&nbsp;provide&nbsp;high&nbsp;accuracy,&nbsp;they&nbsp;can&nbsp;be&nbsp;time-consuming&nbsp;and&nbsp;costly.&nbsp;In&nbsp;contrast,&nbsp;microbial&nbsp;biosensors&nbsp;sensitive&nbsp;to&nbsp;toxic&nbsp;compounds&nbsp;offer&nbsp;alternative&nbsp;approaches&nbsp;that&nbsp;enable&nbsp;rapid&nbsp;and&nbsp;on-site&nbsp;detection&nbsp;of&nbsp;environmental&nbsp;pollutants.</p><p>In&nbsp;this&nbsp;context,&nbsp;the&nbsp;aim&nbsp;of&nbsp;this&nbsp;study&nbsp;was&nbsp;to&nbsp;determine&nbsp;the&nbsp;bioluminescence-based&nbsp;responses&nbsp;to&nbsp;the&nbsp;pesticides&nbsp;cyprodinil,&nbsp;chlorpropham&nbsp;and&nbsp;chlorpyrifos,&nbsp;which&nbsp;are&nbsp;commonly&nbsp;used&nbsp;in&nbsp;agricultural&nbsp;areas,&nbsp;and&nbsp;to&nbsp;evaluate&nbsp;the&nbsp;applicability&nbsp;of&nbsp;these&nbsp;responses&nbsp;in&nbsp;biosensor&nbsp;applications.&nbsp;Within&nbsp;this&nbsp;scope,&nbsp;bioluminescent&nbsp;bacteria&nbsp;were&nbsp;isolated&nbsp;from&nbsp;marine&nbsp;samples&nbsp;and&nbsp;the&nbsp;obtained&nbsp;isolates&nbsp;were&nbsp;molecularly&nbsp;identified&nbsp;based&nbsp;on&nbsp;the&nbsp;16S&nbsp;rDNA&nbsp;gene&nbsp;region&nbsp;and&nbsp;determined&nbsp;to&nbsp;belong&nbsp;to&nbsp;the&nbsp;genus&nbsp;<em>Vibrio</em>.&nbsp;Among&nbsp;the&nbsp;isolated&nbsp;bioluminescent&nbsp;bacteria,&nbsp;the&nbsp;strain&nbsp;showing&nbsp;the&nbsp;most&nbsp;pronounced&nbsp;bioluminescence&nbsp;response,&nbsp;<em>Vibrio</em>&nbsp;sp.&nbsp;SL-2,&nbsp;was&nbsp;selected&nbsp;and&nbsp;the&nbsp;experimental&nbsp;studies&nbsp;were&nbsp;continued&nbsp;with&nbsp;this&nbsp;strain.&nbsp;The&nbsp;selected&nbsp;strain&nbsp;was&nbsp;exposed&nbsp;to&nbsp;different&nbsp;concentrations&nbsp;(100&nbsp;ppm,&nbsp;10&nbsp;ppm,&nbsp;1&nbsp;ppm&nbsp;and&nbsp;0.01&nbsp;ppm)&nbsp;of&nbsp;cyprodinil,&nbsp;chlorpropham&nbsp;and&nbsp;chlorpyrifos,&nbsp;and&nbsp;the&nbsp;resulting&nbsp;bioluminescence&nbsp;responses&nbsp;were&nbsp;analyzed&nbsp;using&nbsp;a&nbsp;digital&nbsp;imaging&nbsp;system&nbsp;in&nbsp;a&nbsp;dark&nbsp;environment&nbsp;and&nbsp;a&nbsp;luminometer&nbsp;device.&nbsp;The&nbsp;results&nbsp;showed&nbsp;that&nbsp;the&nbsp;SL-2&nbsp;strain&nbsp;exhibited&nbsp;distinct&nbsp;and&nbsp;dose-dependent&nbsp;bioluminescence&nbsp;responses&nbsp;to&nbsp;each&nbsp;of&nbsp;the&nbsp;tested&nbsp;pesticides,&nbsp;particularly&nbsp;at&nbsp;concentrations&nbsp;of&nbsp;100ppm&nbsp;and&nbsp;10&nbsp;ppm.&nbsp;These&nbsp;findings&nbsp;indicate&nbsp;that&nbsp;this&nbsp;strain&nbsp;may&nbsp;serve&nbsp;as&nbsp;a&nbsp;potential&nbsp;biological&nbsp;indicator&nbsp;for&nbsp;biosensor-based&nbsp;applications&nbsp;that&nbsp;can&nbsp;be&nbsp;used&nbsp;in&nbsp;environmental&nbsp;monitoring&nbsp;studies&nbsp;due&nbsp;to&nbsp;its&nbsp;sensitive&nbsp;and&nbsp;measurable&nbsp;bioluminescence&nbsp;responses&nbsp;to&nbsp;pesticides.</p>

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