Development of Effective Film Systems for the Treatment of Cold Sores Caused by Herpes Simplex Virus

Project no. 29726 · Status: Kapanmış (Yayın bekleniyor) · 2023

Summary

<p><span style="color: black;">This&nbsp;project&nbsp;aimed&nbsp;to&nbsp;develop&nbsp;film&nbsp;systems&nbsp;to&nbsp;support&nbsp;the&nbsp;alleviation&nbsp;of&nbsp;symptoms&nbsp;of&nbsp;herpes&nbsp;simplex&nbsp;virus-induced&nbsp;cold&nbsp;sores.&nbsp;Therefore,&nbsp;polymeric&nbsp;film&nbsp;formulations&nbsp;were&nbsp;prepared&nbsp;using&nbsp;chitosan,&nbsp;sodium&nbsp;alginate,&nbsp;and&nbsp;pullulan&nbsp;polymers&nbsp;to&nbsp;create&nbsp;a&nbsp;mechanical&nbsp;barrier&nbsp;in&nbsp;the&nbsp;lesion&nbsp;area&nbsp;and&nbsp;increase&nbsp;patient&nbsp;compliance.&nbsp;Additionally,&nbsp;ursolic&nbsp;acid,&nbsp;which&nbsp;has&nbsp;antioxidant&nbsp;and&nbsp;antimicrobial&nbsp;effects,&nbsp;was&nbsp;added&nbsp;to&nbsp;the&nbsp;formulations.&nbsp;The&nbsp;prepared&nbsp;films&nbsp;were&nbsp;characterized&nbsp;in&nbsp;terms&nbsp;of&nbsp;macroscopic&nbsp;properties,&nbsp;thickness,&nbsp;weight,&nbsp;pH,&nbsp;moisture&nbsp;content,&nbsp;dispersion&nbsp;time,&nbsp;water&nbsp;retention&nbsp;capacity,&nbsp;mucoadhesion,&nbsp;mechanical&nbsp;strength,&nbsp;and&nbsp;microscopic&nbsp;morphology.&nbsp;Furthermore,&nbsp;sterilization,&nbsp;microbiological&nbsp;control,&nbsp;antimicrobial&nbsp;efficacy,&nbsp;antioxidant&nbsp;capacity,&nbsp;biocompatibility,&nbsp;and&nbsp;stability&nbsp;studies&nbsp;were&nbsp;conducted.&nbsp;</span></p><p><span style="color: black;">The&nbsp;results&nbsp;showed&nbsp;that&nbsp;pullulan-based&nbsp;film&nbsp;formulations&nbsp;exhibited&nbsp;more&nbsp;favorable&nbsp;properties&nbsp;compared&nbsp;to&nbsp;other&nbsp;formulations&nbsp;in&nbsp;terms&nbsp;of&nbsp;appearance,&nbsp;flexibility,&nbsp;homogeneity,&nbsp;mucoadhesion,&nbsp;and&nbsp;stability&nbsp;under&nbsp;normal&nbsp;storage&nbsp;conditions.&nbsp;Ursolic&nbsp;acid,&nbsp;possessing&nbsp;antimicrobial&nbsp;and&nbsp;antioxidant&nbsp;activity,&nbsp;was&nbsp;added&nbsp;to&nbsp;the&nbsp;formulation.&nbsp;Antioxidant&nbsp;activity&nbsp;was&nbsp;observed&nbsp;in&nbsp;formulations&nbsp;containing&nbsp;ursolic&nbsp;acid,&nbsp;but&nbsp;no&nbsp;significant&nbsp;antimicrobial&nbsp;activity&nbsp;was&nbsp;detected&nbsp;under&nbsp;the&nbsp;tested&nbsp;conditions.&nbsp;However,&nbsp;microbial&nbsp;quality&nbsp;studies&nbsp;showed&nbsp;that&nbsp;ursolic&nbsp;acid-loaded&nbsp;formulations&nbsp;were&nbsp;microbiologically&nbsp;protected.&nbsp;The&nbsp;cytotoxicity&nbsp;of&nbsp;the&nbsp;products&nbsp;was&nbsp;evaluated&nbsp;in&nbsp;two&nbsp;different&nbsp;studies.&nbsp;In&nbsp;addition,&nbsp;studies&nbsp;conducted&nbsp;within&nbsp;the&nbsp;scope&nbsp;of&nbsp;biocompatibility&nbsp;showed&nbsp;that&nbsp;the&nbsp;selected&nbsp;pullulan-based&nbsp;formulation&nbsp;was&nbsp;suitable&nbsp;in&nbsp;terms&nbsp;of&nbsp;dermal&nbsp;irritation&nbsp;and&nbsp;sensitization.&nbsp;In&nbsp;conclusion,&nbsp;the&nbsp;developed&nbsp;film&nbsp;systems&nbsp;are&nbsp;promising&nbsp;for&nbsp;an&nbsp;innovative&nbsp;medical&nbsp;device&nbsp;approach&nbsp;in&nbsp;the&nbsp;management&nbsp;of&nbsp;herpes&nbsp;lesions.&nbsp;Herein,&nbsp;an&nbsp;innovative&nbsp;product&nbsp;has&nbsp;been&nbsp;developed&nbsp;to&nbsp;alleviate&nbsp;herpes&nbsp;symptoms,&nbsp;prevent&nbsp;the&nbsp;spread&nbsp;of&nbsp;herpes&nbsp;to&nbsp;surrounding&nbsp;tissues,&nbsp;and&nbsp;contribute&nbsp;to&nbsp;the&nbsp;healing&nbsp;process&nbsp;of&nbsp;lesions.</span></p>

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