Determination of Antimicrobial Resistance of Staphylococcus aureus Against Secondary Metabolites
Project no. 1367 · Status: Kapanmış · 2017
Summary
In this study, the growth behavior of Staphylococcus aeureus ATCC 6538 strain was investigated in the presence of secondary metabolites. As secondary metabolites, ß-carotene and ß-cryptoxhantin from the carotenoid group, rutine and catechol from the phenolic acid group, and a fatty acid mixture from the fatty acid group was selected as model components. The specific growth behavior in the presence of metabolites was compared with Escherichia coli ATCC 25922 strain. Strain resistance for single, binary and ternary mixtures of secondary metabolites was determined at different concentrations. The anti-inflammatory activity of the algal extract containing carotenoid, phenolic acid and fatty acids was determined and the activity of the extract was investigated according to antimicrobial susceptibility tests. According to the experimental plan created using the surface response method, the antimicrobial resistance change was determined according to the secondary metabolite presence parameters and the effect of the presence of multiple metabolites on growth and the outputs were modelled. As a result a synergistic effect was observed. The most effective combination of compounds was found as ß-cryptoxhantin, rutine and fatty acid mixture with 3.62 µM EC50 concentration. The growth inhibition potential of the algal extract with 100 mg/mL concentration was estimated as %97.2 for S. aureus.