SIMULTANEOUS DETECTION OF GRAPEVINE VIRUSES BY MULTIPLEX REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION
Project no. 22060 · Status: Kapanmış (Yayın bekleniyor) · 2021
Summary
<p>Grapevine viruses pose a significant threat to the viticulture industry, leading to reduced grape yields, lower fruit quality, and economic losses. Accurate and timely detection of multiple viruses is crucial for effective disease management and the implementation of appropriate control measures. In this study, we aimed to assess the presence of Grapevine Fanleaf Virus (GFLV), Grapevine Fleck Virus (GFkV), Grapevine Leafroll-associated Virus-1 (GLRaV-1), Arabis Mosaic Virus (ArMV), and Grapevine Virus A (GVA) in 320 plant samples collected from the AEGEAn region using the powerful technique of Multiplex Reverse Transcription Polymerase Chain Reaction (RT-PCR). Through comprehensive analysis, 119 infected samples were identified out of 320 samples by using Multiplex PCR. The distribution of the detected viruses revealed the presence of 28 GFLV, 31 GLRaV-1, 12 GFkV, 21 GVA, and 27 ArMV viruses. Through comprehensive analysis of the samples, we successfully detected and identified the targeted viruses simultaneously, using a single, cost-effective assay. Our findings revealed the prevalence and distribution of these viruses in the AEGEAn region, providing valuable insights into the viral landscape of grapevines in this important viticultural area. The high detection rate of these viruses emphasizes the urgent need for improved disease management strategies to safeguard grapevine health and productivity. This study holds significant implications for grapevine growers and researchers alike. By enabling the rapid and simultaneous detection of multiple viruses, our findings provide a foundation for effective monitoring, early diagnosis, and targeted disease management. Moreover, the identification of prevalent viruses in the Aegean Region serves as a important reference for future research on virus-host interactions, epidemiology, and the development of region-specific management protocols. The development and application of multiplex RT-PCR as a diagnostic tool for grapevine viruses will contribute to the advancement of precision viticulture, allowing growers to make informed decisions regarding virus control measures, planting strategies, and the selection of virus-resistant cultivars. Ultimately, the implementation of such measures will lead to improved grape quality, enhanced vineyard sustainability, and increased profitability for the viticulture industry.</p>