ETERMINATION OF FEED VALUES OF SOME ALTERNATIVE PROTEIN SOURCES USED IN NUTRITION OF RUMINANTS BY IN VITRO GAS PRODUCTION TECHNIQUE (HOHENHEIM FEED TEST)

Project no. 22676 · Status: Kapanmış (Yayın bekleniyor) · 2021

Summary

DETERMINATION OF FEED VALUES OF SOME ALTERNATIVE PROTEIN SOURCES USED IN NUTRITION OF RUMINANTS BY IN VITRO GAS PRODUCTION TECHNIQUE (HOHENHEIM FEED TEST) ABSTRACT This study was carried out in two stages to determine the feed values of some alternative protein source microalgae (Spirulina platensis, Chlorella vulgaris and Schizochytrium spp.) with in vitro gas production technique (Hohenheim Feed Test). In the first stage, the crude nutrient compositions values of the research material microalgae were found by in vitro Weende analysis method. In the second stage, the gas production (GP) amounts of these microalgae were found as a result of the incubation period of 3, 6, 9, 12, 24, 48 and 72 hours with in vitro gas production technique, and then they were incubated using 24-hour gas formation and some crude nutrient contents. IVOMD (In vitro Organic Matter Digestion), ME (Metabolic Energy) and NEL (Net Energy Lactation) values were calculated. In the study, AA (Acetic acid), PA (Propionic acid), BA (Butyric acid), Total (∑) VFA (Volatile fatty acids) and CH4 VFA (Methane volatile fatty acids) were calculated. The mean crude protein contents of Spirulina platensis, Chlorella vulgaris and Schizochytrium spp. microalgae used in the study were 61.69 %, 41.03 and 15.40 in dry matter (DM) respectively. The average gas production amounts of the same microalgae for 3 and 72 hours were calculated between 11.17 and 28.23 ml/200mg DM, between 7.35 and 44.54 ml/200mg DM, and between 2.86 and 10.85 ml/200mg DM, respectively. In addition, the mean of IVOMD, ME and NEL values of Spirulina platensis, Chlorella vulgaris and Schizochytrium spp. microalgae were 68.64%, 2464 and 1406 kcal/kg DM for Spirulina platensis; 73.71%, 2442 and 1437 kcal/kg DM for Chlorella vulgaris; 31.10%, 1935 and 1010 kcal/kg DM for Schizochytrium spp. were found. In addition, the mean ∑VFA, CH4VFA values of Spirulina platensis, Chlorella vulgaris and Schizochytrium spp. used in the study respectively; 3.33 mg/mL, 1.13 mol/mol VFA for Spirulina platensis; 5.72 mg/mL, 1.86 mol/mol VFA for Chlorella vulgaris; Schizochytrium spp. for 2.37 mg/mL, 0.70 mol/mol VFA were found. In conclusion, when microalgae as crude protein alternative topic respectively from largest to smallest Spirulina platensis, Chlorella vulgaris and Schizochytrium spp.; crude cellulose, which is an important factor in the formation of methane, according to the 24-hour gas production amounts, ME, NE and CH4 VFA values, Chlorella vulgaris, Spirulina platensis, and Schizochytrium spp. were found to be different from largest to smallest, respectively. As an alternative protein source in ruminant nutrition, respectively; it can be said that Chlorella vulgaris, Spirulina platensis can be used, whereas it is more advantageous to use Schizochytrium spp. as a feed additive because it affects rumen methane suppression and reduction.Keywords: Microalgae, feed value, in vitro, methane.

View details