Determination of Biochemical Contents of Microalgae Species Cultured at Different Nitrogen Concentrations
Project no. 22737 · Status: Kapanmış · 2021
Summary
<p>In this study, the growth and biochemical composition changes of Anabaena subcylindirica, Chlamydomonas reinhardtii, Chlorella vulgaris, Dunaliella tertiolecta, Nannochloropsis oculata, Phaeodactylum tricornutum, Spirulina platensis, Tetraselmis chuii, Tetraselmis suecica, and Tisochrysis lutea species were examined in both optimal and nitrogen-deprived culture conditions. Nitrogen deficiency was found to slow down the growth rate in microalgae species and cause alterations in their pigment, carbohydrate, protein, lipid contents, and fatty acid profiles. The study revealed that these species are rich in polyunsaturated fatty acids (PUFA), and particularly, the PUFA levels in P. tricornutum, T. chuii, and T. suecica species increased under nitrogen stress. Eicosapentaenoic acid (EPA) (C20:5) levels were observed to rise in D. tertiolecta, N. oculata, P. tricornutum, T. chuii, and T. suecica species under nitrogen-deficient conditions. Additionally, D. tertiolecta was observed to increase its carbohydrate content by 59% under nitrogen deprivation. P. tricornutum, under nitrogen stress, increased its lipid accumulation by 139% and triacylglycerol (TAG) content by 7.4 times compared to the control group. Furthermore, the potential applications of the studied microalgae species under nitrogen-deficient conditions in various sectors were evaluated. Keywords: Microalgae, Nitrogen deprivation, Stress response, Biochemical composition, Fatty acid profile </p>