ENCAPSULATION OF RESVERATROL IN COMPOSITE BIOPOLYMER NANOPARTICLES
Project no. 21282 · Status: Kapanmış (Yayın bekleniyor) · 2020
Summary
<p>In recent years, nanoparticles produced from biopolymers and biosurfactants have been widely used in controlled delivery systems. The scope of this study includes the preparation of complex composite nanoparticles from biopolymer zein and biosurfactant rhamnolipid and the encapsulation of resveratrol polyphenol into produced capsules. It was aimed to develop a methodology to obtain a promising controlled delivery system with resveratrol loaded zein-rhamnolipid composite nanoparticles. Thus, an efficient encapsulation and release system for the bioactive compound resveratrol has been developed to overcome environmental challenges and prevent diseases. In this study, organic solvent-free and environmentally friendly pHdriven encapsulation method was used to decrease cost and energy of the process. Resveratrol-free nanocapsules were prepared by pH-driven method using solutions of both edible biomolecule zein and biosurfactant rhamnolipid at basic and acidic pH conditions, respectively. The most appropriate zein-rhamnolipid mass ratio was determined as 1:0.6/Z:RL nanoparticles by UV-Visible analysis. Resveratrol (15, 30, 70, 120 and 160 mg) was encapsulated into the produced 1:0.6/Z:RL NPs. The optimum resveratrol-loaded nanocapsules (Z-RL-120Res) had a resveratrol concentration of 0.6 mg/ml. Encapsulation efficiency of 87.35 % - 98.96 % and loading x capacity of 0.83 % - 8.95 % were obtained from the Res-loaded NPs. Nanocapsules were characterized by ATR-FTIR spectroscopy, UV-Vis spectrophotometer and Scanning Electron Microscopy (SEM) analyses. Particle size distribution (DLS) and zeta potential measurements were also performed. In stability control, the capsules were found to be substantially stable over a wide range of pH values (3-9), salt concentrations (0-400 mM), and resveratrol amounts (0-160 mg). Controlled release studies were carried out with the optimum Z-RL-120Res NPs at pH 1.2 and 7.4 using ultrasonic and shaking water bath. Both release studies under acidic and neutral pH conditions demonstrated first rapid release, then slowing down and finally sustained release profiles in phosphate buffer solutions. The Z-RL-120Res NPs had the highest release rates of 89.27 % and 82.24 % at pH 1.2 and 7.4, respectively, in the ultrasonic bath. Moreover, release rates of 79.79 % and 77.22 % were obtained for Z-RL-120Res in the shaking water bath at pH 1.2 and 7.4, respectively. Among the release models, the Gompertz model was the best fitting model for the Z-RL-15Res and Z-RL-160Res NPs. On the other hand, the Weibull model best fitted to the Z-RL-30Res, Z-RL-70Res and Z-RL-120Res NPs. In this study, nanoparticles prepared by pH-driven method had high encapsulation efficiency, loading capacity and stability, which provided better delivery and release control. Characterization results indicated that the resveratrol bioactive compound could be efficiently incorporated into dietary functional products. Consequently, a promising biopolymer-based delivery method could be developed for use in the pharmaceutical and food industry by loading bioactive resveratrol into zeinrhamnolipid nanoparticles. </p>